Induced pluripotent stem cell-derived vascular smooth muscle cells: methods and application

Dash, Biraja C.; Zhengxin Jiang; Suh, Carol; Yibing Qyang
January 2015
Biochemical Journal;1/15/2015, Vol. 465 Issue 2, p185
Academic Journal
Vascular smooth muscle cells (VSMCs) play a major role in the pathophysiology of cardiovascular diseases. The advent of induced pluripotent stem cell (iPSC) technology and the capability of differentiating into virtually every cell type in the human body make this field a ray of hope for vascular regenerative therapy and understanding of the disease mechanism. In the present review, we first discuss the recent iPSC technology and vascular smooth muscle development from an embryo and then examine different methodologies to derive VSMCs from iPSCs, and their applications in regenerative therapy and disease modelling.


Related Articles

  • Give your heart a chance: match the muscle to the vessel. Andreadis, Stelios T. // Cardiovascular Research;Apr2013, Vol. 98 Issue 1, p1 

    No abstract available.

  • INDUCED PLURIPOTENT STEM CELLS: HOW THEY WILL CHANGE THE PRACTICE OF CARDIOVASCULAR MEDICINE. Wing Tak Wong; Sayed, Nazish; Cooke, John P. // Methodist DeBakey Cardiovascular Journal;Oct-Dec2013, Vol. 9 Issue 4, p206 

    Induced pluripotent stem cells (iPSCs) can be generated from adult somatic tissues by the forced expression of a few defined transcription factors, including Oct4, Sox2, Klf4, and c-Myc. iPSC technology holds tremendous promises for therapeutic cardiovascular regeneration because of the cells'...

  • A Time Course Analysis of the Electrophysiological Properties of Neurons Differentiated from Human Induced Pluripotent Stem Cells (iPSCs). Prè, Deborah; Nestor, Michael W.; Sproul, Andrew A.; Jacob, Samson; Koppensteiner, Peter; Chinchalongporn, Vorapin; Zimmer, Matthew; Yamamoto, Ai; Noggle, Scott A.; Arancio, Ottavio // PLoS ONE;Jul2014, Vol. 9 Issue 7, p1 

    Many protocols have been designed to differentiate human embryonic stem cells (ESCs) and human induced pluripotent stem cells (iPSCs) into neurons. Despite the relevance of electrophysiological properties for proper neuronal function, little is known about the evolution over time of important...

  • Contents Page.  // Cardiovascular Research;Nov2013, Vol. 100 Issue 2, pNP 

    No abstract available.

  • MicroRNAs in Vascular Biology. Munekazu Yamakuchi // International Journal of Vascular Medicine;2012, p1 

    Vascular inflammation is an important component of the pathophysiology of cardiovascular diseases, such as hypertension, atherosclerosis, and aneurysms. All vascular cells, including endothelial cells (ECs) and vascular smooth muscle cells (VSMCs), and infiltrating cells, such as macrophages,...

  • Functional vascular smooth muscle cells derived from human induced pluripotent stem cells via mesenchymal stem cell intermediates. Bajpai, Vivek K.; Mistriotis, Panagiotis; Loh, Yuin-Han; Daley, George Q.; Andreadis, Stelios T. // Cardiovascular Research;Dec2012, Vol. 96 Issue 3, p391 

    Aims Smooth muscle cells (SMC) play an important role in vascular homeostasis and disease. Although adult mesenchymal stem cells (MSC) have been used as a source of contractile SMC, they suffer from limited proliferation potential and culture senescence, particularly when originating from older...

  • Regulation of Endothelial Nitric Oxide Synthase in Pathophysiological Conditions. Isenović, Esma R.; Soskić, Sanja; Düngen, Hans-Dirk; Dobutović, Branislava; Elvis, Tahirović; Simone, Inkrot; Marche, Pierre // Cardiovascular & Haematological Disorders - Drug Targets;Sep2011, Vol. 11 Issue 2, p109 

    The nitric oxide (NO) cascade and endothelial NO synthase (eNOS) are best known for their role in endothelium-mediated relaxation of vascular smooth muscle (VSM). NO generated by eNOS has been established as a key regulatory signaling molecule in the vasculature. The activities of eNOS are...

  • Transcriptional and post-transcriptional regulation of cGMP-dependent protein kinase (PKG-I): pathophysiological significance. Sellak, Hassan; Choi, Chung-sik; Dey, Nupur B.; Lincoln, Thomas M. // Cardiovascular Research;Feb2013, Vol. 97 Issue 2, p200 

    The ability of the endothelium to produce nitric oxide, which induces generation of cyclic guanosine monophosphate (cGMP) that activates cGMP-dependent protein kinase (PKG-I), in vascular smooth muscle cells (VSMCs), is essential for the maintenance of vascular homeostasis. Yet, disturbance of...

  • Enhancing Induced Pluripotent Stem CellModels of Schizophrenia. Wright, Rebecca; Réthelyi, János M.; Gage, Fred H. // JAMA Psychiatry;Mar2014, Vol. 71 Issue 3, p334 

    The article discusses issues related to enhancing induced pluripotent stem cell (IPSC) models of schizophrenia. It cites several ways to enhance future IPSC models of schizophrenia to reduce variability between studies. It also examines the generation of specific, homogeneous neuronal...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics