TITLE

GDNF and NT-3 Induce Progenitor Bone Mesenchymal Stem Cell Differentiation into Neurons in Fetal Gut Culture Medium

AUTHOR(S)
Zhu, Tianqi; Yu, Donghai; Feng, Jiexiong; Wu, Xiaojuan; Xiang, Lei; Gao, Heyun; Zhang, Xueqin; Wei, Mingfa
PUB. DATE
March 2015
SOURCE
Cellular & Molecular Neurobiology;Mar2015, Vol. 35 Issue 2, p255
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
With the increasing use of bone marrow mesenchymal stem cells (BMSCs) in cell therapies, factors regulating BMSC differentiation have become the interest of current research. In this study, we investigated the effects of glial cell-derived neurotrophic factor (GDNF) and neurotrophin-3 (NT-3) on the course of BMSC differentiation. BMSCs were isolated from rat bone marrow and transfected with GDNF and NT-3 genes. Compared to mock-transfected BMSCs, GDNF and NT-3 induced BMSC differentiation to reveal neuron-like characteristics, i.e., the positive expression of neuronal marker MAP-2 and astrocyte marker GFAP, as detected by immunofluorescence assays. Semi-quantitative polymerase chain reaction (PCR) and western blot analyses showed that the increase of expression of GDNF and NT-3 in BMSCs also simultaneously elevated the mRNA expression of NSE, nestin, and MAP-2. Furthermore, the cell patch-clamp test demonstrated that the overexpression of GDNF and NT-3 in BMSCs enhanced voltage-activated potassium currents, implying that BMSCs possess great potential as a cell-based therapeutic candidate to treat neurological diseases.
ACCESSION #
101113657

 

Related Articles

  • Efficient Gene Therapy for Parkinson's Disease Using Astrocytes as Hosts for Localized Neurotrophic Factor Delivery. Drinkut, Anja; Tereshchenko, Yuliya; Schulz, Jörg B; Bähr, Mathias; Kügler, Sebastian // Molecular Therapy;Mar2012, Vol. 20 Issue 3, p534 

    Current gene therapy approaches for Parkinson's disease (PD) deliver neurotrophic factors like glial cell line-derived neurotrophic factor (GDNF) or neurturin via neuronal transgene expression. Since these potent signaling-inducing neurotrophic factors can be distributed through long-distance...

  • GDNF Overexpression from the Native Locus Reveals its Role in the Nigrostriatal Dopaminergic System Function. Kumar, Anmol; Kopra, Jaakko; Varendi, Kärt; Porokuokka, Lauriina L.; Panhelainen, Anne; Kuure, Satu; Marshall, Pepin; Karalija, Nina; Härma, Mari-Anne; Vilenius, Carolina; Lilleväli, Kersti; Tekko, Triin; Mijatovic, Jelena; Pulkkinen, Nita; Jakobson, Madis; Jakobson, Maili; Ola, Roxana; Palm, Erik; Lindahl, Maria; Strömberg, Ingrid // PLoS Genetics;12/17/2015, Vol. 11 Issue 12, p1 

    Degeneration of nigrostriatal dopaminergic system is the principal lesion in Parkinson’s disease. Because glial cell line-derived neurotrophic factor (GDNF) promotes survival of dopamine neurons in vitro and in vivo, intracranial delivery of GDNF has been attempted for Parkinson’s...

  • Targeted Delivery of GDNF through the Blood-Brain Barrier by MRI-Guided Focused Ultrasound. Feng Wang; Yu Shi; Lin Lu; Li Liu; Youli Cai; Hairong Zheng; Xin Liu; Fei Yan; Chao Zou; Chengyu Sun; Jie Shi; Shukun Lu; Yun Chen // PLoS ONE;Dec2012, Vol. 7 Issue 12, p1 

    Neurotrophic factors, such as glial cell line-derived neurotrophic factor (GDNF), are promising therapeutic agents for neurodegenerative diseases. However, the application of GDNF to treat these diseases effectively is limited because the blood-brain barrier (BBB) prevents the local delivery of...

  • ANTIHYPOXIC AND NEUROPROTECTIVE PROPERTIES OF BDNF AND GDNF in vitro AND in vivo UNDER HYPOXIC CONDITIONS. Vedunova, M. V.; Sakharnova, T. A.; Mitroshina, E. V.; Shishkina, T. V.; Astrakhanova, T. A.; Mukhina, I. V. // Medical Technologies in Medicine / Sovremennye Tehnologii v Medi;2014, Vol. 6 Issue 4, p38 

    The aim of the investigation was to assess antihypoxic and neuroprotective properties of the brain-derived neurotrophic factor (BDNF) and the glial cell line-derived neurotrophic factor (GDNF) during in vitro and in vivo hypoxia models. Materials and Methods. In vitro studies were performed...

  • GDNF Family Ligands: A Potential Future for Parkinson's Disease Therapy. Mickiewicz, Amanda L.; Kordower, Jeffrey H. // CNS & Neurological Disorders - Drug Targets;Sep2011, Vol. 10 Issue 6, p703 

    Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by motor dysfunction that occurs secondary to loss of dopaminergic neurons in the nigrostriatal pathway. Current pharmacotherapies focus on the replacement of lost dopamine to alleviate disease symptoms. However,...

  • Non-viral gene therapy for GDNF production in RCS rat: the crucial role of the plasmid dose. Touchard, E; Heiduschka, P; Berdugo, M; Kowalczuk, L; Bigey, P; Chahory, S; Gandolphe, C; Jeanny, J-C; Behar-Cohen, F // Gene Therapy;Sep2012, Vol. 19 Issue 9, p886 

    Glial cell line-derived neurotrophic factor (GDNF) is one of the candidate molecules among neurotrophic factors proposed for a potential treatment of retinitis pigmentosa (RP). It must be administered repeatedly or through sustained releasing systems to exert prolonged neuroprotective effects....

  • Upregulation of p-Akt by glial cell line-derived neurotrophic factor ameliorates cell apoptosis in the hippocampus of rats with streptozotocin-induced diabetic encephalopathy. WEIGANG CUI; YINGHUA ZHANG; DERONG LU; MINGXIN REN; GUOYAN YUAN // Molecular Medicine Reports;2016, Vol. 13 Issue 1, p543 

    The loss of neurotrophic factor support has been shown to contribute to the development of the central nervous system. Glial cell line-derived neurotrophic factor (GDNF), a potent neurotrophic factor, is closely associated with apoptosis and exerts neuroprotective effects on numerous populations...

  • Neuron-Type Specific Functions of DNT1, DNT2 and Spz at the Drosophila Neuromuscular Junction. Sutcliffe, Ben; Forero, Manuel G.; Zhu, Bangfu; Robinson, Iain M.; Hidalgo, Alicia // PLoS ONE;Oct2013, Vol. 8 Issue 10, p1 

    Retrograde growth factors regulating synaptic plasticity at the neuromuscular junction (NMJ) in Drosophila have long been predicted but their discovery has been scarce. In vertebrates, such retrograde factors produced by the muscle include GDNF and the neurotrophins (NT: NGF, BDNF, NT3 and NT4)....

  • Influence of Lithium Treatment on GDNF Serum and CSF Concentrations in Patients with Early Alzheimer's Disease. Straten, Guido; Saur, Ralf; Laske, Christoph; Gasser, Thomas; Annas, Peter; Basun, Hans; Leyhe, Thomas // Current Alzheimer Research;Dec2011, Vol. 8 Issue 8, p853 

    Preclinical and clinical studies gave evidence that lithium could be useful in the treatment of Alzheimer's disease (AD). One possible mechanism of action might be the induction of neurotrophins. Recently, we found a significant increase of brain-derived neurotrophic factor (BDNF) serum levels...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics