Transplantation of Autologous Bone Marrow Mesenchymal Stem Cells with Platelet-Rich Plasma Accelerate Distraction Osteogenesis in A Canine Model

Dehghan, Mohammad Mehdi; Eslaminejad, Mohamadreza Baghaban; Motallebizadeh, Nader; Halan, Javad Ashrafi; Tagiyar, Leila; Soroori, Sarang; Nikmahzar, Agbibi; Pedram, Mirsepehr; Shahverdi, Abdolhossein; Mehrjerdi, Hossein Kazemi; Izadi, Sadra
June 2015
Cell Journal (Yakhteh);Summer2015, Vol. 17 Issue 2, p243
Academic Journal
Objective: Distraction osteogenesis (DO) is a surgical procedure used to generate large volumes of new bone for limb lengthening. Materials and Methods: In this animal experimental study, a 30% lengthening of the left tibia (mean distraction distance: 60.8 mm) was performed in ten adult male dogs by callus distraction after osteotomy and application of an llizarov fixator. Distraction was started on postoperative day seven with a distraction rate of 0.5 mm twice per day and carried out at a rate of 1.5 mm per day until the end of the study. Autologous bone marrow mesenchymal stem cells (BM-MSCs) and platelet-rich plasma (PRP) as the treatment group (n=5) or PRP alone (control group, n=5) were injected into the distracted callus at the middle and end of the distraction period. At the end of the consolidation period, the dogs were sacrificed after which computerized tomography (CT) and histomorphometric evaluations were performed. Results: Radiographic evaluationsrevealed that the amount and quality of callus formations were significantly higher in the treatment group (P<0.05). As measured by CT scan, the healing parametersin dogs of the treatment group were significantly greater (P<0.05). New bone formation in the treatment group was significantly higher (P<0.05). Conclusion: The present study showed that the transplantation of BM-MSCs positively affects early bony consolidation in DO. The use of MSCs might allow a shortened period of consolidation and therefore permit earlier device removal.


Related Articles

  • External fixation of femoral defects in athymic rats: Applications for human stem cell implantation and bone regeneration. Foo, Terasa; Reagan, Jeffrey; Watson, John T.; Moed, Berton R.; Zijun Zhang // Journal of Tissue Engineering;Jan-Dec2013, Vol. 4, p1 

    An appropriate animal model is critical for the research of stem/progenitor cell therapy and tissue engineering for bone regeneration in vivo. This study reports the design of an external fixator and its application to critical-sized femoral defects in athymic rats. The external fixator consists...

  • Simvastatin Induces Osteogenic Differentiation of Murine Embryonic Stem Cells. Pagkalos, Joseph; Jae Min Cha; Yunyi Kang; Heliotis, Manolis; Tsiridis, Eleftherios; Mantalaris, Athanasios // Journal of Bone & Mineral Research;Nov2010, Vol. 25 Issue 11, p2470 

    The article discusses research on osteogenic differentiation of murine embryonic stem cells (mESCs) that can be induced by simvastatin. The researchers conducted in vitro studies on bone marrow stromal cells and ESCs where statins were seen to have cholesterol-independent anabolic effects on the...

  • Effects of Initial Cell Density and Hydrodynamic Culture on Osteogenic Activity of Tissue-Engineered Bone Grafts. Fei Luo; Tian-Yong Hou; Ze-Hua Zhang; Zhao Xie; Xue-Hui Wu; Jian-Zhong Xu // PLoS ONE;Jan2013, Vol. 8 Issue 1, Special section p1 

    This study aimed to study the effects of initial cell density and in vitro culture method on the construction of tissue-engineered bone grafts and osteogenic activities. Human mesenchymal stem cells (hMSCs) were seeded onto cubic scaffolds prepared from demineralized bone matrix (DBM) by three...

  • Dexamethasone induces osteogenesis via regulation of hedgehog signalling molecules in rat mesenchymal stem cells. Ma, Xiaoli; Zhang, Xueping; Jia, Yanfei; Zu, Shanshan; Han, Shuyi; Xiao, Dongjie; Sun, Haiji; Wang, Yunshan // International Orthopaedics;Jul2013, Vol. 37 Issue 7, p1399 

    Purpose: Hedgehog signalling plays an important role during the development of tissues and organs, including bone and limb. Dexamethasone (DEX), a synthetic and widely used glucocorticoid, affects osteogenesis of bone marrow mesenchymal stem cells (MSCs), while the signalling pathway by which...

  • The Role of Systemically Delivered Bone Marrow-Derived Mesenchymal Stem Cells in the Regeneration of Periodontal Tissues. Meijiao Yu; Shaohua Ge; Fang Wang; Yong Wen; Xinlong Yan; Quan Zeng; Wen Yue; Pishan Yang; Xuetao Pei // Oral & Craniofacial Tissue Engineering;2011, Vol. 1 Issue 4, p331 

    Purpose: Recent studies have shown that periodontal ligament stem cells (PDLSCs) play a key role in periodontal regeneration. However, the origin of these cells remains unclear. Meanwhile, bone marrow is thought to be the most common source of adult stem cells in many tissues and organs. Thus,...

  • Cell Therapy of Refractory Crohn’s Disease. Knyazev, O. V.; Parfenov, A. I.; Shcherbakov, P. L.; Ruchkina, I. N.; Konoplyannikov, A. G. // Bulletin of Experimental Biology & Medicine;Nov2013, Vol. 156 Issue 1, p139 

    We analyzed medium-term efficiency and safety of biological therapy of Crohn’s disease, in particular transplantation of allogenic mesenchymal stromal bone marrow cells and anticytokine therapy with selective immunosuppressive agents. It was found that both methods of biological therapy...

  • Hypoxic Preconditioning Increases Survival and Pro-Angiogenic Capacity of Human Cord Blood Mesenchymal Stromal Cells In Vitro. Bader, Andreas Matthäus; Klose, Kristin; Bieback, Karen; Korinth, Dirk; Schneider, Maria; Seifert, Martina; Choi, Yeong-Hoon; Kurtz, Andreas; Falk, Volkmar; Stamm, Christof // PLoS ONE;9/18/2015, Vol. 10 Issue 9, p1 

    Hypoxic preconditioning was shown to improve the therapeutic efficacy of bone marrow-derived multipotent mesenchymal stromal cells (MSCs) upon transplantation in ischemic tissue. Given the interest in clinical applications of umbilical cord blood-derived MSCs, we developed a specific hypoxic...

  • Clinical and radiographic characterization of xenotransplantation of rat bone marrow-derived mesenchymal stem cells for repair of radial defects of rabbit. MONTEIRO, Betânia; FAVARATO, Lukiya; CARVALHO, Pablo; OKANO, Barbara; MENEGATTI, Maria Antônia; OLIVEIRA, Alvaro; SANTOS, Bianka; DEL CARLO, Ricardo // Turkish Journal of Veterinary & Animal Sciences;2015, Vol. 39 Issue 5, p511 

    This study aimed to evaluate the clinical and radiographic aspects of xenogenic transplantation of rat bone marrow-derived mesenchymal stem cells (MSCs) for the repair of radial bone defects of rabbit. Bone marrow derived MSCs collected from Wistar rats were expanded, differentiated, and labeled...

  • Treating Achilles Tendon Rupture in Rats with Bone-Marrow-Cell Transplantation Therapy. Okamoto, Naofumi; Kushida, Taketoshi; Oe, Kenichi; Umeda, Masayuki; Ikehara, Susumu; Iida, Hirokazu // Journal of Bone & Joint Surgery, American Volume;12/1/2010, Vol. 92-A Issue 17, p2776 

    Background: Bone marrow cells possess multipotentiality and have been used for several treatments. We hypothesized that bone marrow cells might differentiate into regenerated tendon and that several cytokines within bone marrow cells might accelerate tendon healing. Therefore, we treated...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics