TITLE

miR-128 represses L1 retrotransposition by binding directly to L1 RNA

AUTHOR(S)
Hamdorf, Matthias; Idica, Adam; Zisoulis, Dimitrios G; Gamelin, Lindsay; Martin, Charles; Sanders, Katie J; Pedersen, Irene M
PUB. DATE
October 2015
SOURCE
Nature Structural & Molecular Biology;Oct2015, Vol. 22 Issue 10, p824
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Long interspersed element 1 (LINE-1 or L1) retrotransposons compose 17% of the human genome. Active L1 elements are capable of replicative transposition (mobilization) and can act as drivers of genetic diversity. However, this mobilization is mutagenic and may be detrimental to the host, and therefore it is under strict control. Somatic cells usually silence L1 activity by DNA methylation of the L1 promoter. In hypomethylated cells, such as cancer cells and induced pluripotent stem cells (iPSCs), a window of opportunity for L1 reactivation emerges, and with it comes an increased risk of genomic instability and tumorigenesis. Here we show that miR-128 represses new retrotransposition events in human cancer cells and iPSCs by binding directly to L1 RNA. Thus, we have identified and characterized a new function of microRNAs: mediating genomic stability by suppressing the mobility of endogenous retrotransposons.
ACCESSION #
110141455

 

Related Articles

  • Induced Pluripotent Mesenchymal Stromal Cell Clones Retain Donor-derived Differences in DNA Methylation Profiles. Shao, Kaifeng; Koch, Carmen; Gupta, Manoj K; Lin, Qiong; Lenz, Michael; Laufs, Stephanie; Denecke, Bernd; Schmidt, Manfred; Linke, Matthias; Hennies, Hans C; Hescheler, Jürgen; Zenke, Martin; Zechner, Ulrich; Šarić, Tomo; Wagner, Wolfgang // Molecular Therapy;Jan2013, Vol. 21 Issue 1, p240 

    Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is an epigenetic phenomenon. It has been suggested that iPSC retain some tissue-specific memory whereas little is known about interindividual epigenetic variation. We have reprogrammed mesenchymal stromal cells from human...

  • Stem cells: The quest for the perfect reprogrammed cell. Krupalnik, Vladislav; Hanna, Jacob H. // Nature;7/10/2014, Vol. 511 Issue 7508, p160 

    The article discusses the problems of molecular defects in the methods for reprogramming mature cells to pluripotent stem cells including derivation of induced pluripotent stem (iPS) cells and somatic cell nuclear transfer (SCNT). Topics discussed include iPS cells causing epignetic differences...

  • Zfp296 Is a Novel, Pluripotent-Specific Reprogramming Factor. Fischedick, Gerrit; Klein, Diana C.; Wu, Guangming; Esch, Daniel; Höing, Susanne; Han, Dong Wook; Reinhardt, Peter; Hergarten, Kerstin; Tapia, Natalia; Schöler, Hans R.; Sterneckert, Jared L. // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    Expression of the four transcription factors Oct4, Sox2, Klf4, and c-Myc (OSKM) is sufficient to reprogram somatic cells into induced pluripotent stem (iPSCs). However, this process is slow and inefficient compared with the fusion of somatic cells with embryonic stem cells (ESCs), indicating...

  • Epigenetics: Methylation reboot. Seton-Rogers, Sarah // Nature Reviews Cancer;May2013, Vol. 13 Issue 5, p292 

    The article discusses research being done on DNA methylation. It references the study "Widespread resetting of DNA methylation in glioblastoma-initiating cells suppresses malignant cellular behavior in a lineage-dependent manner," by S. H. Stricker and colleagues in the 2013 issue. It adds that...

  • Direct reprogramming of adult cells: avoiding the pluripotent state. Kelaini, Sophia; Cochrane, Amy; Margariti, Andriana // Stem Cells & Cloning: Advances & Applications;2014, Vol. 7, p19 

    The procedure of using mature, fully differentiated cells and inducing them toward other cell types while bypassing an intermediate pluripotent state is termed direct reprogramming. Avoiding the pluripotent stage during cellular conversions can be achieved either through ectopic expression of...

  • Dynamic and static maintenance of epigenetic memory in pluripotent and somatic cells. Shipony, Zohar; Mukamel, Zohar; Cohen, Netta Mendelson; Landan, Gilad; Tanay, Amos; Chomsky, Elad; Zeliger, Shlomit Reich; Friedman, Nir; Fried, Yael Chagit; Ainbinder, Elena // Nature;9/4/2014, Vol. 513 Issue 7516, p115 

    Stable maintenance of gene regulatory programs is essential for normal function in multicellular organisms. Epigenetic mechanisms, and DNA methylation in particular, are hypothesized to facilitate such maintenance by creating cellular memory that can be written during embryonic development and...

  • A comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs. Choi, Jiho; Lee, Soohyun; Mallard, William; Clement, Kendell; Tagliazucchi, Guidantonio Malagoli; Lim, Hotae; Choi, In Young; Ferrari, Francesco; Tsankov, Alexander M; Pop, Ramona; Lee, Gabsang; Rinn, John L; Meissner, Alexander; Park, Peter J; Hochedlinger, Konrad // Nature Biotechnology;Nov2015, Vol. 33 Issue 11, p1173 

    The equivalence of human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) remains controversial. Here we use genetically matched hESC and hiPSC lines to assess the contribution of cellular origin (hESC vs. hiPSC), the Sendai virus (SeV) reprogramming method and...

  • Use of UTF1 Genetic Control Elements as iPSC Reporter. Morshedi, Amir; Soroush Noghabi, Monireh; Dröge, Peter // Stem Cell Reviews & Reports;Aug2013, Vol. 9 Issue 4, p523 

    The reprogramming of adult somatic cells into an embryonic stem cell (ESC) state by various means has opened a new chapter in basic and applied life science. While this technology will create great opportunities for regenerative medicine, the more immediate impact is likely to be found in human...

  • Reprogramming cancer cells: back to the future. Lang, J-Y; Shi, Y; Chin, Y E // Oncogene;5/2/2013, Vol. 32 Issue 18, p2247 

    Reprogramming healthy somatic cells into induced pluripotent stem cells (iPSCs) with four defined factors (Oct4, Sox2, c-Myc and Klf4) has been intensively investigated. However, reprogramming diseased cells such as cancer cells has fallen much behind. In this issue of Oncogene, Zhang et al....

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics