Clinical and radiographic characterization of xenotransplantation of rat bone marrow-derived mesenchymal stem cells for repair of radial defects of rabbit

MONTEIRO, Betânia; FAVARATO, Lukiya; CARVALHO, Pablo; OKANO, Barbara; MENEGATTI, Maria Antônia; OLIVEIRA, Alvaro; SANTOS, Bianka; DEL CARLO, Ricardo
September 2015
Turkish Journal of Veterinary & Animal Sciences;2015, Vol. 39 Issue 5, p511
Academic Journal
This study aimed to evaluate the clinical and radiographic aspects of xenogenic transplantation of rat bone marrow-derived mesenchymal stem cells (MSCs) for the repair of radial bone defects of rabbit. Bone marrow derived MSCs collected from Wistar rats were expanded, differentiated, and labeled in the laboratory. In the fourth passage, the cultures were pelleted in aliquots of 107 cells/50 µL and transplanted in situ into the lesions. Thirty-six rabbits were used in the in vivo study. In all animals, defects were created by removing a bone segment of 1.0 cm in length in the right radius. The rabbits were divided into four experimental groups: the control group (CG) received percutaneously administered 50 µL of phosphate buffer (PBS) in the radial bone defect region 24 h after lesion creation; group 1 (G1) had the radial bone defect filled with absorbable gelatin sponge soaked with 50 μL of PBS during the surgery; group 2 (G2) had the radial bone defect filled with absorbable gelatin sponge soaked with the MSC pellet; and group 3 (G3) received percutaneously the MSC pellet in the radial bone defect region 24 h after lesion creation. No clinical and radiographic changes consistent with cellular rejection were observed. Percutaneous MSC xenotransplantation contributed to the repair of experimental radial bone defects.


Related Articles

  • Cell Therapy of Refractory Crohn’s Disease. Knyazev, O. V.; Parfenov, A. I.; Shcherbakov, P. L.; Ruchkina, I. N.; Konoplyannikov, A. G. // Bulletin of Experimental Biology & Medicine;Nov2013, Vol. 156 Issue 1, p139 

    We analyzed medium-term efficiency and safety of biological therapy of Crohn’s disease, in particular transplantation of allogenic mesenchymal stromal bone marrow cells and anticytokine therapy with selective immunosuppressive agents. It was found that both methods of biological therapy...

  • Optimization of adipose tissue-derived mesenchymal stem cells by rapamycin in a murine model of acute graft-versus-host disease. Kyoung-Woon Kim; Su-Jin Moon; Min-Jung Park; Bo-Mi Kim; Eun-Kyung Kim; Sung-Hee Lee; Eun-Jung Lee; Byung-Ha Chung; Chul-Woo Yang; Mi-La Cho // Stem Cell Research & Therapy;10/23/2015, Vol. 6, p1 

    Introduction: Mesenchymal stem cells (MSCs) can protect bone marrow transplantation (BMT) recipients from the lethal acute graft-versus-host disease (aGVHD) development. However, the mechanisms underlying the anti-inflammatory properties of MSCs in aGVHD remain to be elucidated. The...

  • The Role of Systemically Delivered Bone Marrow-Derived Mesenchymal Stem Cells in the Regeneration of Periodontal Tissues. Meijiao Yu; Shaohua Ge; Fang Wang; Yong Wen; Xinlong Yan; Quan Zeng; Wen Yue; Pishan Yang; Xuetao Pei // Oral & Craniofacial Tissue Engineering;2011, Vol. 1 Issue 4, p331 

    Purpose: Recent studies have shown that periodontal ligament stem cells (PDLSCs) play a key role in periodontal regeneration. However, the origin of these cells remains unclear. Meanwhile, bone marrow is thought to be the most common source of adult stem cells in many tissues and organs. Thus,...

  • Transplantation of Autologous Bone Marrow Mesenchymal Stem Cells with Platelet-Rich Plasma Accelerate Distraction Osteogenesis in A Canine Model. Dehghan, Mohammad Mehdi; Eslaminejad, Mohamadreza Baghaban; Motallebizadeh, Nader; Halan, Javad Ashrafi; Tagiyar, Leila; Soroori, Sarang; Nikmahzar, Agbibi; Pedram, Mirsepehr; Shahverdi, Abdolhossein; Mehrjerdi, Hossein Kazemi; Izadi, Sadra // Cell Journal (Yakhteh);Summer2015, Vol. 17 Issue 2, p243 

    Objective: Distraction osteogenesis (DO) is a surgical procedure used to generate large volumes of new bone for limb lengthening. Materials and Methods: In this animal experimental study, a 30% lengthening of the left tibia (mean distraction distance: 60.8 mm) was performed in ten adult male...

  • Hypoxic Preconditioning Increases Survival and Pro-Angiogenic Capacity of Human Cord Blood Mesenchymal Stromal Cells In Vitro. Bader, Andreas Matthäus; Klose, Kristin; Bieback, Karen; Korinth, Dirk; Schneider, Maria; Seifert, Martina; Choi, Yeong-Hoon; Kurtz, Andreas; Falk, Volkmar; Stamm, Christof // PLoS ONE;9/18/2015, Vol. 10 Issue 9, p1 

    Hypoxic preconditioning was shown to improve the therapeutic efficacy of bone marrow-derived multipotent mesenchymal stromal cells (MSCs) upon transplantation in ischemic tissue. Given the interest in clinical applications of umbilical cord blood-derived MSCs, we developed a specific hypoxic...

  • Treating Achilles Tendon Rupture in Rats with Bone-Marrow-Cell Transplantation Therapy. Okamoto, Naofumi; Kushida, Taketoshi; Oe, Kenichi; Umeda, Masayuki; Ikehara, Susumu; Iida, Hirokazu // Journal of Bone & Joint Surgery, American Volume;12/1/2010, Vol. 92-A Issue 17, p2776 

    Background: Bone marrow cells possess multipotentiality and have been used for several treatments. We hypothesized that bone marrow cells might differentiate into regenerated tendon and that several cytokines within bone marrow cells might accelerate tendon healing. Therefore, we treated...

  • Patient presents with chronic KCS, corneal thinning. Mayo, George L.; Starck, Tomy; Johnson, Daniel A. // Ophthalmology Times;04/15/2001, Vol. 26 Issue 8, p18 

    Presents the case of a Hispanic man with 4A non-Hodgkin's lymphoma, previously treated with chemoradiotherapy and allogeneic bone marrow cell transplantation (BMT). Details on the medical examination of the patient; Possibilities that could account for the initial corneal epithelial defect of...

  • Neural stem cell-like cells derived from autologous bone mesenchymal stem cells for the treatment of patients with cerebral palsy. Guojun Chen; Yali Wang; Zhenyu Xu; Feng Fang; Renmei Xu; Yue Wang; Xiaoli Hu; Lixing Fan; Houqi Liu // Journal of Translational Medicine;2013, Vol. 11 Issue 1, p1 

    Background: Stem cell therapy is a promising treatment for cerebral palsy, which refers to a category of brain diseases that are associated with chronic motor disability in children. Autologous MSCs may be a better cell source and have been studied for the treatment of cerebral palsy because of...

  • The beneficial effects of intracoronary autologous bone marrow stem cell transfer as an adjunct to percutaneous coronary intervention in patients with acute myocardial infarction. Wang, Xiang; Xi, Wei-chun; Wang, Fang // Biotechnology Letters;Nov2014, Vol. 36 Issue 11, p2163 

    The efficacy of post-percutaneous coronary intervention (PCI) intracoronary injection with bone marrow mesenchymal stem cells (BMSCs) in patients with acute myocardial infarction (AMI) remains controversial. Here, 58 patients with AMI undergoing PCI were randomly divided into two groups: BMSC...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics