Role of bone morphogenetic protein-2 in osteogenic differentiation of mesenchymal stem cells

September 2015
Molecular Medicine Reports;2015, Vol. 12 Issue 3, p4230
Academic Journal
Bone mesenchymal stem cells (BMSCs) have been an area of interest in biomedical research and tissue engineering due to their diverse differentiation abilities. In osteogenesis, bone morphogenetic proteins (BMPs), particularly BMP-2, are important. However, the effect of BMP-2 on the osteogenetic capacity of BMSCs remains to be fully elucidated. In the present study, primary rat BMSCs were infected with a recombinant lentivirus carrying the BMP-2 gene (Lenti-BMP-2), and the effects of BMP-2 on the activity of alkaline phosphatase (ALP) on days 3, 7, 14 and 21, and on mineralization on day 21 were evaluated. In addition, the adhesive ability of BMP-2-overexpressed BMSCs was detected using an adhesion assay. Following forced expression of BMP-2 in the BMSCs, the levels of osteogenic genes, including osteopontin (OPN), osteocalcin (OC) and collagen type I (Col-I), were detected and the nuclear accumulation of Runt-related transcription factor (Runx)-2 and phosphorylated small mothers against decapentaplegic (p-Smad) 1/5/8 was also evaluated. The results demonstrated that the rat BMSCs had been isolated, cultured and passaged from Sprague-Dawley rat bone marrow successfully, and the third-generation BMSCs were identified using flow cytometry with CD29 staining. The osteogenetic phenotype of the BMSCs, expressing ALP and osteocalcin, was significantly induced by BMP-2, and the proliferation of the BMSCs was enhanced by BMP-2. Furthermore, the adhesive potential of the BMP-2-overexpressed BMSCs was increased, the expression levels of OPN, OCN and Col-Ie osteogenetic factors were upregulated and the nuclear accumulation of Runx-2 and p-Smads1/5/8 were increased significantly. These data suggested that BMP-2 may facilitate the osteogenetic differentiation of rat BMSCs and provide a favorable cell resource for tissue engineering.


Related Articles

  • Inhibition of Histone Deacetylases Potentiates BMP9-Induced Osteogenic Signaling in Mouse Mesenchymal Stem Cells. Hu, Ning; Wang, Changdong; Liang, Xi; Yin, Liangjun; Luo, Xiaoji; Liu, Bo; Zhang, Hongyu; Shui, Wei; Nan, Guoxin; Wang, Ning; Wu, Ningning; Chen, Xian; He, Yunfeng; Wen, Sheng; Deng, Fang; Zhang, Hongmei; Liao, Zhan; Luu, Hue H.; Haydon, Rex C.; He, Tong-Chuan // Cellular Physiology & Biochemistry (Karger AG);Sep2013, Vol. 32 Issue 2, p486 

    Background/Aims: We have demonstrated that bone morphogenetic protein 9 (BMP9) is one of the most potent BMPs in regulating osteoblast differentiation of mesenchymal stem cells (MSCs) although the molecular mechanism underlying BMP9-induced osteogenesis remains to be fully elucidated. It is...

  • The effect of nano-scale topography on osteogenic differentiation of mesenchymal stem cells. Faghihi, Faezeh; Eslaminejad, Mohamadreza Baghaban // Biomedical Papers of the Medical Faculty of Palacky University i;Mar2014, Vol. 158 Issue 1, p5 

    Background. Large bone defects resulting from trauma or disease pose a threat to humans. Thus far, tissue engineering as an important clinical approach uses cells, growth factors and scaffolds to regenerate large areas of damaged bone tissue. Since bone is a nanocomposite structure, it is...

  • Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells. JIANYI NIU; GANG DING; LI ZHANG // Molecular Medicine Reports;2015, Vol. 12 Issue 6, p8237 

    The present study aimed to investigate the effects of simvastatin on the bone differentiation capacity and immunological characteristics of bone marrow mesenchymal stem cells (BMSCs). BMSCs were isolated and cultured in medium containing 1.0 μmol/ml simvastatin. The alkaline phosphatase...

  • Terbium promotes adhesion and osteogenic differentiation of mesenchymal stem cells via activation of the Smad-dependent TGF-β/BMP signaling pathway. Liu, Dan-Dan; Ge, Kun; Jin, Yi; Sun, Jing; Wang, Shu-Xiang; Yang, Meng-Su; Zhang, Jin-Chao // Journal of Biological Inorganic Chemistry;Aug2014, Vol. 19 Issue 6, p879 

    With its special physical and chemical properties, terbium has been widely used, which has inevitably increased the chance of human exposure to terbium-based compounds. It was reported that terbium mainly deposited in bone after introduction into the human body. Although some studies revealed...

  • Osteogenic differentiation potential of canine bone marrowderived mesenchymal stem cells under different β-glycerophosphate concentrations in vitro. Sawangmake, Chenphop; Nantavisai, Sirirat; Osathanon, Thanaphum; Pavasant, Prasit // Thai Journal of Veterinary Medicine;2016, Vol. 46 Issue 4, p617 

    Stem cell-based therapy employing bone marrow-derived mesenchymal stem cells has been proposed as a promising approach for bone regenerative treatment. Despite numerous studies supporting the application in human medicine, such information in veterinary practice is still lacking. Thus, this...

  • MiR-125b Regulates the Osteogenic Differentiation of Human Mesenchymal Stem Cells by Targeting BMPR1b. Wang, Huaqing; Xie, Zhao; Hou, Tianyong; Li, Zhiqiang; Huang, Ke; Gong, Jicheng; Zhou, Wei; Tang, Kanglai; Xu, Jianzhong; Dong, Shiwu // Cellular Physiology & Biochemistry (Karger AG);May2017, Vol. 41 Issue 2, p530 

    Background/Aims: Osteogenic differentiation of mesenchymal stem cells (MSCs) plays a crucial role in bone regeneration and bone reparation. This complex process is regulated precisely and firmly by specific factors. Recent studies have demonstrated that miR-125b regulates osteogenic...

  • Time-Dependent Expression of Bone Sialoprotein Fragments in Osteogenesis Induced by Bone Morphogenetic Protein1. Kobayashi, Daiji; Takita, Hiroko; Mizuno, Morimichi; Toteuka, Yasunori; Kuboki, Yoshlnori // Journal of Biochemistry;1996, Vol. 119 Issue 3, p475 

    Bone sialoprotein is unique to bone and dentin, but its precise role in these tissues is still unknown, although several hypotheses have been presented. We chose ectopic chondro- and osteogenesis induced by bone morphogenetic protein (BMP) as a model system to examine the role of this...

  • Clinical characteristics influence in vitro action of 1,25-dihydroxyvitamin D3 in human marrow stromal cells. Zhou, Shuanhu; Glowacki, Julie; Kim, Sung Won; Hahne, Jochen; Geng, Shuo; Mueller, Stefan M; Shen, Longxiang; Bleiberg, Ilan; LeBoff, Meryl S // Journal of Bone & Mineral Research;Sep2012, Vol. 27 Issue 9, p1992 

    Vitamin D is important for bone health, with low vitamin D levels being associated with skeletal fragility and fractures. Among its other biological activities, 1,25-dihydroxyvitamin D (1,25(OH)2D), stimulates the in vitro differentiation of human marrow stromal cells (hMSCs) to osteoblasts,...

  • Pore Size of Porous Hydroxyapatite as the Cell-Substratum Controls BMP-Induced Osteogenesis1. Tsuruga, Eichi; Takita, Hiroko; Itoh, Hideaki; Wakisaka, Yuichi; Kuboki, Yoshinori // Journal of Biochemistry;1997, Vol. 121 Issue 2, p317 

    To elucidate the biochemical mechanism of osteogenesis, the effect of matrix geometry upon the osteogenesis induced by bone morphogenetic protein (BMP) was studied. A series of five porous hydroxyapatites with different pore sizes, 106–212, 212–300, 300–400, 400–500,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics