TITLE

Properties of Phenylalnine Ammonialyase in Dioscorea opposita 'Tiegun' and Its Browning Control

AUTHOR(S)
Xiting ZHAO; Liwei JIANG; Yannan LI; Lin MA; Huizhen WANG; Mingjun LI
PUB. DATE
August 2015
SOURCE
Medicinal Plant;Aug2015, Vol. 6 Issue 7/8, p34
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
With Diascorea opposita 'Tiegun' as the research materials, phenylalnine ammonialyase (PAL) eharaeteristies related to browning and the effects of different inhibitors on PAL activity were studied by spectrophotometry. Research results showed that within the test range, the optimal pH was 8.6 for PAL of D. opposita 'Tiegun'. PAL had poor tolerance to acid and alkali. The optimal temperature was 50 °C, showing certain tolerance to high temperature. Even under incubation for 30 min at 80 °C, there were still alxiut 12% PAL activity. L-eysteine hvdro-ehloride and citric acid had significant inhibitory effects on PAL; while phytic acid had no significant inhibitory effects on PAL activity; but phytic acid showed no significant inhibitory effects. Scxlium chloride promoted the PAL activity. In order to effectively restrict the browning of fresh slices of D. opposita 'Tiegun' caused by PAL, processing and storage of D. opposita 'Tiegun' slices shoidd be carried out under acidic condition (pH < 2.0). low temperature (< 20 °C ) or high temperature (>80 °C). L-eysteine hydrochloride should be the first choice of and browning reagent without sulfite for fresh slices, followed with citric acid.
ACCESSION #
110696077

 

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