Expansion of mesenchymal adipose-tissue derived stem cells in a stirred single-use bioreactor under low-serum conditions

Schirmaier, Carmen; Kaiser, Stephan C.; Jossen, Valentin; Brill, Silke; Jüngerkes, Frank; van den Bos, Christian; Eibl, Dieter; Eibl, Regine
December 2013
BMC Proceedings;12/4/2013, p1
Academic Journal
The article presents a study which discusses expansion of mesenchymal adipose-tissue derived stem cells in a single-use bioreactor under low-serum conditions. Topics discussed include increase in demand of human mesenchymal stem cells (hMSCs), use of preliminary tests in disposable-spinners to determine microcarrier (MC) types for serum reduced expansions, and flow cytometry tests revealing marker profiles following cell expansion and harvest.


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