TITLE

Expression Analysis of 14-3-3 Gene in Tall Fescue under Several Abiotic Stresses

AUTHOR(S)
Xiaodong LI; Erru YU; Jianhong SHU; Jiahai WU; Xiaoli WANG
PUB. DATE
October 2015
SOURCE
Agricultural Science & Technology;Oct2015, Vol. 16 Issue 10, p2207
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
To study the functions of 14-3-3 gene family in tall fescue, the potential functions of 13 14-3-3 proteins in Arabidopsis were investigated by bioinformatic analysis. Based on the sequences of 14-3-3 genes in tall fescue by transcriptome and proteomic sequencing, the full-length cDNA sequences of 4 14-3-3 genes in tall fescue were obtained. Their sequences were aligned by Clustal W2. The results showed that the genetic relationships between 14-3-3A and 14-3-3D, 14-3-3B and 14-3-3C are closer, and their main structures are very conservative. The changes in expression levels of 14-3-3 genes under low nitrogen, drought, high temperature and high salt stresses were investigated by fluorescence quantitative PCR. The expression level of 14-3-3A makes responses to low nitrogen, drought, high temperature and high salt stresses; the expression levels of other genes also make responses to abiotic stresses in varying degrees, but the relevant response mechanisms are not exactly the same. Therefore, it is speculated that the 14-3-3 gene family regulates stress resistance of plants through different pathways, and functional differentiation occurs during its evolution.
ACCESSION #
111815829

 

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