TITLE

Susceptibility to Lipopolysaccharide of Cholestatic Rat Liver Produced with Bile Duct Ligation: Assessments of the Mitochondrial Glutathione Pool and the Effects of N-Acetylcysteine

AUTHOR(S)
Nakano, H.; Fujiwara, Y.; Kitamura, N.; Kumada, K.; Matsumiya, A.; Sakai, H.; Hatakeyama, T.; Yamaguchi, M.; Jaeck, D.
PUB. DATE
May 2000
SOURCE
European Surgical Research;2000, Vol. 32 Issue 3, p148
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We investigated whether rats with obstructive jaundice produced with bile duct ligation for 2 weeks are more susceptible to the additional stress of lipopolysaccharide (LPS) administration than sham-operated rats and also examined the effects of N-acetylcysteine (NAC) on LPS stimulation in rats with bile duct ligation. The effects of LPS on the mitochondrial glutathione pool and on oxidative stress of polymorphonuclear leukocytes were investigated in cholestatic rats. Serum concentrations of α-glutathione S-transferase showed that lipopolysaccharide stimulation caused more severe hepatocellular injury in cholestatic rats than in sham-operated rats. In addition, concentrations of mitochondrial reduced and oxidized glutathione and hepatic adenosine triphosphate showed that LPS stimulation decreased mitochondrial function more in cholestatic rats than in sham-operated rats. Intraperitoneal administration of NAC for 2 weeks significantly improved mitochondrial function and decreased hepatocellular injury. However, the oxidative stress of polymorphonuclear leukocytes that had infiltrated hepatic tissue was increased by NAC. The present results indicate that the cholestatic liver is susceptible to the additional stress of LPS, that NAC suppresses the adverse effects of LPS in cholestatic livers, and that the oxidative stress of polymorphonuclear leukocytes is not significantly involved in mitochondrial dysfunction or hepatocellular injury in this model.Copyright © 2000 S. Karger AG, Basel
ACCESSION #
11375448

 

Related Articles

  • Fucoxanthin restrains oxidative stress induced by retinol deficiency through modulation of Na+Ka+-ATPase and antioxidant enzyme activities in rats. Ravi Kumar, Sangeetha; Narayan, Bhaskar; Vallikannan, Baskaran // European Journal of Nutrition;Dec2008, Vol. 47 Issue 8, p432 

    Retinol deficiency is a major public health problem world wide, affecting children and women, in particular. It causes a variety of disorders in the body affecting various cellular functions. To study the effect of fucoxanthin (FUCO), a non-provitamin-A carotenoid in comparison with retinol...

  • Antioxidant activities of dithiol alpha-lipoic acid. Islam, M. T. // Bangladesh Journal of Medical Science;Jun2009, Vol. 8 Issue 3, p6 

    Alpha-lipoic acid, a dithiol compound derived from octanoic acid, which acts as a coenzyme for several redox reactions in almost all the tissue of the body. It retains its protective functions in both oxidized and reduced forms. Alpha-lipoic acid reduces oxidative stress by redox generation of...

  • Mimicking of glutathione peroxidase deficiency by exposition of JAR cells to increased level of synthetic hydroperoxide. Hallmann, Anna; Milczarek, Ryszard; Szkatuła, Michał; Woźniak, Michał; Spodnik, Jan Henryk; Klimek, Jerzy // Folia Morphologica;2005, Vol. 64 Issue 4, p304 

    A short chain synthetic analogue of lipid hydroperoxides was used to overload glutathione peroxidase (GPx) in human choriocarcinoma cell line JAR cells. Cells exposed to 100 μM tBuOOH displayed a 40% reduction in ATP level and significantly increased in membrane permeability, visualised by...

  • Structure and Antioxidant Catalytic Function of Plant Glutathione Transferases. Chronopoulou, Evangelia; Axarli, Irene; Nianiou-Obeidat, Irini; Madesis, Panagiotis; Tsaftaris, Athanasios; Labrou, Nikolaos E. // Current Chemical Biology;Jan2011, Vol. 5 Issue 1, p64 

    Plant cytosolic glutathione transferases (GSTs) are an ancient enzyme superfamily with multiple and diverse functions which are important in counteracting biotic and abiotic stresses. GSTs play an important role in catalyzing the conjugation of xenobiotics and endogenous electrophilic compounds...

  • Tolerance of Transplastomic Tobacco Plants Overexpressing a Theta Class Glutathione Transferase to Abiotic and Oxidative Stresses. Stavridou, Evangelia; Michailidis, Michail; Gedeon, Stella; Ioakeim, Antri; Kostas, Stefanos; Chronopoulou, Evangelia; Labrou, Nikolaos E.; Edwards, Robert; Day, Anil; Nianiou-Obeidat, Irini; Madesis, Panagiotis // Frontiers in Plant Science;1/11/2019, pN.PAG 

    Chloroplasts are organelles subjected to extreme oxidative stress conditions. Biomolecules produced in the chloroplasts act as signals guiding plant metabolism toward stress tolerance and play a major role in regulating gene expression in the nucleus. Herein, we used transplastomic plants as an...

  • The mitochondrial transporter ABC-me (ABCB10), a downstream target of GATA-1, is essential for erythropoiesis in vivo. Hyde, B B; Liesa, M; Elorza, A A; Qiu, W; Haigh, S E; Richey, L; Mikkola, H K; Schlaeger, T M; Shirihai, O S // Cell Death & Differentiation;Jul2012, Vol. 19 Issue 7, p1117 

    The mitochondrial transporter ATP binding cassette mitochondrial erythroid (ABC-me/ABCB10) is highly induced during erythroid differentiation by GATA-1 and its overexpression increases hemoglobin production rates in vitro. However, the role of ABC-me in erythropoiesis in vivo is unknown. Here we...

  • Effect of diallyldisulphide on an antioxidant enzyme system in Candida species. Yousuf, Snowber; Ahmad, Aijaz; Khan, Amber; Manzoor, Nikhat; Khan, Luqman Ahmad // Canadian Journal of Microbiology;Oct2010, Vol. 56 Issue 10, p816 

    This study was carried out to show the effect of diallyldisulphide (DADS), an important organosulphur compound found in garlic (Allium sativum), on antioxidant systems in Candida species. Changes in antioxidant metabolites and antioxidant activity in the presence of DADS were found in Candida...

  • Opposite effects of GSTM1 - and GSTT1 - gene deletion variants on bone mineral density. Mlakar, Simona Jurkovic; Osredkar, Josko; Prezelj, Janez; Marc, Janja // Disease Markers;2011, Vol. 31 Issue 5, p279 

    Oxidative stress is associated with osteoporosis. The glutathione S-transferases form the major detoxifying group of enzymes responsible for eliminating products of oxidative stress. We have therefore proposed GSTM1 and GSTT1 genes as candidates for studying the genetics of osteoporosis. The aim...

  • Protective Effect of Rutin Against Cadmium Induced Hepatotoxicity in Swiss Albino Mice. Mirani, Nagma; Ashraf, Nagma Jamal; Siddique, Jamal; Rub, Abdur // Journal of Pharmacology & Toxicology;2012, Vol. 7 Issue 3, p150 

    Cadmium is an extremely toxic metal which has no known necessary function in the body. Industrial use and agricultural fertilizers are the major source of its environmental contamination. It principally affects lung, liver, kidney and testes following acute intoxication. The present study...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics