TITLE

Expression of a Codon-Optimized dsdA Gene in Tobacco Plastids and Rice Nucleus Confers D-Serine Tolerance

AUTHOR(S)
Yanmei Li; Rui Wang; Zongliang Hu; Hongcai Li; Shizhan Lu; Juanjuan Zhang; Yongjun Lin; Fei Zhou; Li, Guotian; Podishetty, Natraj Kumar; Rogalski, Marcelo
PUB. DATE
May 2016
SOURCE
Frontiers in Plant Science;5/12/2016, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
D-serine is toxic to plants. D-serine ammonia lyase, which is encoded by the dsdA gene, can attenuate this toxicity with high specificity. In the present study, we explored the function of codon-optimized dsdA with tobacco plastids and rice nuclear transformation system. It was shown that dsdA gene was site-specifically integrated into the tobacco plastid genome and displayed a high level of expression. Genetic analysis of the progenies showed that dsdA gene is maternally inherited and confers sufficient D-serine resistance in tobacco. The effective screening concentrations of D-serine for seed germination, callus regeneration and foliar spray were 10, 30, and 75 mM, respectively. In addition, calluses from homozygous transgenic rice lines also showed significant tolerance to D-serine (up to 75 mM). Our study proves the feasibility of using dsdA gene as a selectable marker in both plastid and nuclear transformation systems.
ACCESSION #
115319909

 

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