Study of virulence gene profiles in β-hemolytic Streptococcus sp. strains

Ionescu, Diana; Chifiriuc, Mariana Carmen; Ionescu, Bogdan; Cristea, Violeta; Sacagiu, Bogdan; Duta, Maria; Neacsu, Gabriela; Gilca, Ramona; Gheorghe, Irina; Curutiu, Carmen; Lazar, Veronica
November 2016
Biointerface Research in Applied Chemistry;2016, Vol. 6 Issue 6, p1607
Academic Journal
Infections caused by Streptococcus pyogenes have significant medical importance because of the high number of complications and postinfectious sequelaes. The aim of this study was to analyze the gene profile of streptococcal superantigenic toxins found in Streptococcus pyogenes and ß-hemolytic group C and G Streptococcus strains isolated from throat and nasal exudates. The analysis of the virulence patterns was conducted using 102 β-hemolytic streptococcal strains. The bacterial DNA was extracted and 3 Multiplex PCR assays were carried out. The results showed that the gene most commonly expressed was the speF gene (70.5% of cases) and the smeZ and speI genes had the lowest expression level (2% and respectively 3%). In conclusion, the β-hemolytic streptococcal strains analyzed possessed a rich repertoire of virulence genes showing the high pathogenic potential of streptococcal strains.


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