TITLE

Study of microRNAs targeted Dvl2 on the osteoblasts differentiation of rat BMSCs in hyperlipidemia environment

AUTHOR(S)
Huang, Xin; Wang, Zhifeng; Li, Duoduo; Huang, Zhengfei; Dong, Xiaofei; Li, Chuanhua; Lan, Jing
PUB. DATE
September 2018
SOURCE
Journal of Cellular Physiology;Sep2018, Vol. 233 Issue 9, p6758
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Dishevelled 2 (Dvl-2), a key mediator of the wnt/β-catenin signaling pathway, plays critical roles in osteoblasts differentiation in hyperlipidemia environment. In our previous study, we observed a strong correlation between increased dvl2 expression and decreased new bone formation around implants in a rat hyperlipidemia implant surgery model. However, transcriptional regulation of Dvl2 by microRNAs in this process remains unknown. In the current study, we searched in online database and identified four significantly up-regulated miRNAs, miR-21-5p, miR-29c-3p, miR-138-5p, and miR-351-5p that could potentially regulate Dvl2. Using Western blot and dual-luciferase assays, we confirmed that miR29c-3p suppresses Dvl2 expression by binding to its 3′-UTR. Our results suggest a novel transcriptional regulation mechanism of Dvl2 by miR-29c-3p in osteoblasts differentiation of BMSCs.
ACCESSION #
130010756

 

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