Novel Polysaccharide H-1-2 from Pseudostellaria Heterophylla Alleviates Type 2 Diabetes Mellitus

Zhao-hui Fang; Xian-chun Duan; Jin-dong Zhao; Yuan-jie Wu; Meng-meng Liu
October 2018
Cellular Physiology & Biochemistry (Karger AG);Oct2018, Vol. 49 Issue 3, p1037
Academic Journal
Background/Aims: To investigate the potential therapeutic effect of novel polysaccharide H-1-2 from pseudostellaria heterophylla against type 2 Diabetes Mellitus (T2DM) and elucidate the underling molecular mechanisms. Methods: Relative expression of HIF1α and Sirt1 in T2DM patients was determined via real-time PCR. The direct binding of HIF1α on Sirt1 promoter was validated by ChIP assay. The inhibitory regulation of Sirt1 by HIF1α was analyzed using luciferase reporter assay. The endogenous protein of HIF1α and Sirt1 in response to H-1-2 treatment was quantified by western blotting. The blood glucose, secreted insulin and serous lipid profiles were measured with ELISA kits. Results: We consolidated that HIF1α and Sirt1 was dysregulated in T2DM patients and subjected to H-1-2 modulation. H-1-2 significantly inhibited hypoxia and up-regulated Sirt1 expression in EndoC-βH1 cells. Accordingly, H-1-2 enhanced glucose-stimulation insulin secretion and improved blood glucose and lipid profiles in T2DM cells, and elevated the glucose and insulin tolerance simultaneously. Furthermore, we demonstrated that H-1-2 alleviated T2DM via inhibition of hypoxia and up-regulation of Sirt1 in isolated pancreatic β-cells from T2DM rats. Conclusion: Our data unambiguously demonstrated H-1-2 administration alleviated T2DM by enhancing Sirt1 expression through inhibition of hypoxia.


Related Articles

  • Treating Type 2 Diabetes Mellitus: A Growing Epidemic. Weinstock, Ruth S. // Mayo Clinic Proceedings;Apr2003, Vol. 78 Issue 4, p411 

    Editorial. Introduces articles published in the April 2003 issue of 'Mayo Clinic Proceedings' journal. Treatment of type-2 diabetes mellitus. Prevalence; Importance of early diagnosis for the initiation of aggressive treatment to prevent complications; Glycemic control; Insulin resistance.

  • MULBERRY NATURALLY AIDS BLOOD SUGAR CONTROL. Kiefer, Dale // Life Extension;Sep2004, Vol. 10 Issue 9, p31 

    Explains that mulberry leaves contain substances that reverse insulin resistance. Study in India involving patients with type II diabetes; Observation that patients taking mulberry leaves had significantly lower fasting glucose; Statistically insignificant effect of glibenclamide.

  • Short Insulin Tolerance Test May Determine Thiazolidinediones Treatment in Type 2 Diabetes. Lee, Mi Y.; Koh, Jang H.; Jung, Pil M.; Shim, Myoung S.; Kim, Mi J.; Shin, Jang Y.; Shin, Young G.; Chung, Choon H. // Diabetes;Jun2007 Supplement 1, Vol. 56, pA578 

    The short insulin tolerance test is a simple and reliable method of estimating insulin sensitivity. This study was designed to compare the insulin sensitizing effects of thiazolidinediones (TZDs) on the degree of insulin resistance determined by short insulin tolerance test, Kitt in type 2...

  • Cinnamon: New Applications for An Old Spice. Hawkins, Ernest B. // Natural Pharmacy;Aug2004, Vol. 8 Issue 4, p12 

    Discusses the potential of cinnamon to help the response to insulin in type 2 diabetes. Role of cinnamon in Traditional Chinese Medicine; Regulation of blood sugar levels; Use of cinnamon oil to treat oral candidiasis in patients with acquired immune deficiency syndrome.

  • A Current Update on the Use of Alpha Lipoic Acid in the Management of Type 2 Diabetes Mellitus. Poh, Z. X.; Goh, K. P. // Endocrine, Metabolic & Immune Disorders - Drug Targets;Dec2009, Vol. 9 Issue 4, p392 

    Type 2 Diabetes Mellitus (T2DM) which is characterised by insulin resistance, is closely linked to the triad of glucolipotoxicity, inflammation and oxidative stress. Increased adiposity, leading to increased free fatty acids (FFAs), contributes to insulin resistance by disrupting the signal...

  • Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence. Farino, Zachary J.; Morgenstern, Travis J.; Vallaghe, Julie; Gregor, Nathalie; Donthamsetti, Prashant; Harris, Paul E.; Pierre, Nicolas; Freyberg, Robin; Charrier-Savournin, Fabienne; Javitch, Jonathan A.; Freyberg, Zachary // PLoS ONE;2/5/2016, Vol. 11 Issue 2, p1 

    Direct measurement of insulin is critical for basic and clinical studies of insulin secretion. However, current methods are expensive and time-consuming. We developed an insulin assay based on homogenous time-resolved fluorescence that is significantly more rapid and cost-effective than current...

  • Dual Peroxisome Proliferator - Activated Receptor α/δ Agonist GFT505 Improves Hepatic and Peripheral Insulin Sensitivity in Abdominally Obese Subjects. CARIOU, BERTRAND; HANF, RÉMY; LAMBERT-PORCHERON, STÉPHANIE; ZAÏR, YASSINE; SAUVINET, VALÉRIE; NOËL, BENOIT; FLET, LAURENT; VIDAL, HUBERT; STAELS, BART; LAVILLE, MARTINE // Diabetes Care;Oct2013, Vol. 36 Issue 10, p2923 

    OBJECTIVE--The development of new insulin sensitizers is an unmet need for the treatment of type 2 diabetes. We investigated the effect of GFT505, a dual peroxisome proliferator-activated receptor (PPAR)-a/d agonist, on peripheral and hepatic insulin sensitivity. RESEARCH DESIGN AND...

  • 10. Glucose control: Insulin-based therapies.  // Journal of Endocrinology, Metabolism & Diabetes of South Africa;Jun2012, Vol. 17 Issue 2, pS32 

    The article discusses the use of insulin in treating patients with type 2 diabetes. It cites several studies that provide guidance for the rational use of various insulins in diabetes treatment, and offers recommendations for the initiation of basal insulin and pre-mixed insulin in diabetic...

  • Insulin in type 2 diabetes. Taylor, James R. // Endocrine Today;Feb2010, Vol. 8 Issue 2, p13 

    The article discusses research on the use of insulin in controlling the blood glucose in patients with type 2 diabetes, by Holman and colleagues, published in the 2009 issue of the "New England Journal of Medicine."


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics