TITLE

Evidence for the involvement of cell wall peroxidase in the generation of hydroxyl radicals mediating extension growth

AUTHOR(S)
Liszkay, Anja; Kenk, Barbara; Schopfer, Peter
PUB. DATE
August 2003
SOURCE
Planta;Aug2003, Vol. 217 Issue 4, p658
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Hydroxyl radicals (·OH), produced in the cell wall, are capable of cleaving wall polymers and can thus mediate cell wall loosening and extension growth. It has recently been proposed that the biochemical mechanism responsible for ·OH generation in the cell walls of growing plant organs represents an enzymatic reaction catalyzed by apoplastic peroxidase (POD). This hypothesis was investigated by supplying cell walls of maize (Zea mays L.) coleoptiles and sunflower (Helianthus annuus L.) hypocotyls with external NADH, an artificial substrate known to cause ·OH generation by POD in vitro. The effects of NADH on wall loosening, growth, and ·OH production in vivo were determined. NADH mediates cell wall extension in vitro and in vivo in an H2O2-dependent reaction that shows the characteristic features of POD. NADH-mediated production of ·OH in vivo was demonstrated in maize coleoptiles using electron paramagnetic resonance spectroscopy in combination with a specific spin-trapping reaction. Kinetic properties and inhibitor/activator sensitivities of the ·OH-producing reaction in the cell walls of coleoptiles resembled the properties of horseradish POD. Apoplastic consumption of external NADH by living coleoptiles can be traced back to the superimposed action of two enzymatic reactions, a KCN-sensitive reaction mediated by POD operating in the ·OH-forming mode, and a KCN-insensitive reaction with the kinetic properties of a superoxide-producing plasma-membrane NADH oxidase the activity of which can be promoted by auxin. Under natural conditions, i.e. in the absence of external NADH, this enzyme may provide superoxide (O2·-) (and H2O2 utilized by POD for) ·OH production in the cell wall.
ACCESSION #
15668655

 

Related Articles

  • Fluorescent tags to explore cell wall structure and dynamics. Gonneau, Martine; Höfte, Herman; Vernhettes, Samantha // Frontiers in Plant Science;Jul2012, Vol. 3, p1 

    Plant cell walls are highly dynamic and heterogeneous structures, which vary between cell types, growth stages but also between microdomains within a single cell wall. In this review, we summarize the imaging techniques using fluorescent tags that are currently being used and which should in the...

  • Silica in Plants: Biological, Biochemical and Chemical Studies. Heather A. Currie; Carole C. Perry // Annals of Botany;Dec2007, Vol. 100 Issue 7, p1383 

    Background The incorporation of silica within the plant cell wall has been well documented by botanists and materials scientists; however, the means by which plants are able to transport silicon and control its polymerization, together with the roles of silica in situ, are not fully understood. ...

  • Dynamics of growth and the content of endogenous phytohormones during kidney bean scoto-and photomorphogenesis. Golovatskaya, I. F.; Karnachuk, R. A. // Russian Journal of Plant Physiology;May2007, Vol. 54 Issue 3, p407 

    The dynamics of growth and the contents of free and bound endogenous IAA, gibberellins (GA), cytokinins (zeatin and its riboside), and ABA in kidney bean plants ( Phaseolus vulgaris L., cv. Belozernaya) grown in darkness or in the light was studied. Phytohormones were quantified in...

  • Functions of Xyloglucan in Plant Cells. Hayashi, Takahisa; Kaida, Rumi // Molecular Plant (Oxford University Press / USA);Jan2011, Vol. 4 Issue 1, p17 

    While an increase in the number of xyloglucan tethers between the cellulose microfibrils in plant cell walls increases the walls’ rigidity, the degradation of these tethers causes the walls to loosen. Degradation can occur either through the integration of xyloglucan oligosaccharides due...

  • Cell differentiation, secondary cell-wall formation and transformation of callus tissue of Pinus radiata D. Don. Möller, Ralf; McDonald, Armando G.; Walter, Christian; Harris, Philip J. // Planta;Sep2003, Vol. 217 Issue 5, p736 

    Tracheid and sclereid differentiation was induced in callus cultures of Pinus radiata D. Don by culturing on a basal medium containing activated charcoal but no phytohormones; sclereids differentiated in callus derived from xylem strips, but not in callus derived from hypocotyl segments. The...

  • Plant cell shapes: Modulators and Measurements. Ivakov, Alexander; Persson, Staffan // Frontiers in Plant Science;Oct2013, Vol. 4, p1 

    Plant cell shape, seen as an integrative output, is of considerable interest in various fields, such as cell wall research, cytoskeleton dynamics and biomechanics. In this review we summarise the current state of knowledge on cell shape formation in plants focusing on shape of simple cylindrical...

  • A developmental gradient in the mechanism of K+ uptake during light-stimulated leaf growth in Nicotiana tabacum L. Stiles, Kari A.; McClintick, Anna; van Volkenburgh, Elizabeth // Planta;Aug2003, Vol. 217 Issue 4, p587 

    Light causes growth of dicotyledonous leaves by stimulating proton efflux, cell wall acidification and loosening, and solute accumulation for turgor maintenance. For cells still undergoing cell division at the base of expanding tobacco (Nicotiana tabacum L. cv. Xanthi) leaves, light-stimulated...

  • The auxin influx carrier LAX3 promotes lateral root emergence. Swarup, Kamal; Benková, Eva; Swarup, Ranjan; Casimiro, Ilda; Péret, Benjamin; Yang, Yaodong; Parry, Geraint; Nielsen, Erik; De Smet, Ive; Vanneste, Steffen; Levesque, Mitch P.; Carrier, David; James, Nicholas; Calvo, Vanessa; Ljung, Karin; Kramer, Eric; Roberts, Rebecca; Graham, Neil; Marillonnet, Sylvestre; Patel, Kanu // Nature Cell Biology;Aug2008, Vol. 10 Issue 8, p946 

    Lateral roots originate deep within the parental root from a small number of founder cells at the periphery of vascular tissues and must emerge through intervening layers of tissues. We describe how the hormone auxin, which originates from the developing lateral root, acts as a local inductive...

  • Cell wall metabolism during maturation, ripening and senescence of peach fruit. Brummell, David A.; Cin, Valeriano Dal; Crisosto, Carlos H.; Labavitch, John M. // Journal of Experimental Botany;Sep2004, Vol. 55 Issue 405, p2029 

    Cell wall changes were examined in fruit of a melting flesh peach (Prunus persica L.) allowed to ripen on the tree. Three phases to softening were noted, the first of which began prior to the completion of flesh colour change and an increase in ethylene evolution. Softening in young mature...

Share

Read the Article

Courtesy of NEW JERSEY STATE LIBRARY

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics