TITLE

A novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides

AUTHOR(S)
Velayos, Antonio; Fuentes-Vicente, Mónica; Aguilar-Elena, Raúl; Eslava, Arturo P.; Iturriaga, Enrique A.
PUB. DATE
June 2004
SOURCE
Current Genetics;Jun2004, Vol. 45 Issue 6, p371
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Two Mucor circinelloides structural genes involved in isoprenoid biosynthesis were isolated and characterised. The isoA gene encodes a typical eukaryotic farnesyl diphosphate synthase (EC 2.5.1.10), whereas the isoB gene deduced amino acid sequence shows similarity to fungal medium-chain prenyl diphosphate synthases. By functional complementation in Escherichia coli, the isoB gene product was shown to be a solanesyl diphosphate synthase (EC 2.5.1.11), which is the first fungal enzyme reported having this specificity. In addition, a M. circinelloides one-marker-per-chromosome map was completed by contour-clamped homogeneous electric field localisation of isoA, isoB and three other isoprenoid biosynthesis genes to individual chromosomes.
ACCESSION #
16899841

 

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