Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection

Jin Sik Oh; Dae Sub Song; Jeong Sun Yang; Ju Young Song; Hyoung Joon Moon; Tae Yung Kim; Bong Kyun Park
December 2005
Journal of Veterinary Science;2005, Vol. 6 Issue 4, p349
Academic Journal
An indirect porcine epidemic diarrhea (PED) virus (PEDV) enzyme-linked immunosorbent assay (ELISA) was compared with the serum neutralization (SN) test by testing 46 samples from experimentally infected sows, 73 samples from naïve sows, and 1,024 field sow samples from 48 commercial swine farms of undefined PED status. The SN test and the ELISA were performed using PEDV, KPEDV-9 strain. Viral proteins as a coating antigen of PEDV ELISA were extracted from the cytoplasm of PEDV-infected Vero cells using a non-ionic detergent, Triton X-100, and a simple protocol of PEDV ELISA was followed. The presence of antibodies in these experimental samples was confirmed by SN and ELISA in which the sensitivity of the ELISA was 89.1%, and the corresponding specificity was 94.5%. On testing 1,024 field samples, an overall agreement of 84.2% was generated between the SN and ELISA. This study demonstrates that the PEDV ELISA is a useful serodiagnostic screening test at herd level for detecting swine antibodies against PEDV.


Related Articles

  • Evaluation of two enzyme-linked immunosorbent assays for serodiagnosis of Aleutian mink disease virus infection in mink. Andersson, Anna-Maria; Wallgren, Per // Acta Veterinaria Scandinavica;2013, Vol. 55 Issue 1, p1 

    Background Aleutian disease in mink is caused by infection with Aleutian mink disease virus (AMDV). In Sweden, the infection most commonly causes classical Aleutian disease in which the immune system fails to neutralize the virus and the infection becomes persistent. Diagnosis of AMDV infection...

  • Immunologic parameters during NOTES compared with laparoscopy in a randomized blinded porcine trial. Bingener, Juliane; Krishnegowda, Naveen K.; Michalek, Joel E. // Surgical Endoscopy;Jan2009, Vol. 23 Issue 1, p178 

    It remains unclear if the natural orifice translumenal endoscopic surgery (NOTES) technique is less invasive than laparoscopy. Serum interleukins and peritoneal cellular response have been utilized to support the immunologic difference between open and laparoscopic surgery. We hypothesized that...

  • Assessment of a commercial sandwich ELISA in the diagnosis of aspergillosis in falcons. Arca-Ruibal, B.; Wernery, U.; Zachariah, R.; Bailey, T. A.; Di Somma, A.; Silvanose, C.; McKinney, P. // Veterinary Record: Journal of the British Veterinary Association;4/1/2006, Vol. 158 Issue 13, p442 

    A commercial sandwich ELISA (Platelia Aspergillus EIA, Bio-Rad) developed for the detection of galactomannan, a major cell wall constituent of Aspergillus species, was tested for its efficacy in the diagnosis of aspergillosis in falcons. Ninety serum samples from 50 aspergillosis-positive...

  • Serum Levels of Interleukin-18 Are Elevated in the Subacute Phase of Kawasaki Syndrome. Nomura, Yuichi; Masuda, Kiminori; Maeno, Nobuaki; Yoshinaga, Masao; Kawano, Yoshifumi // International Archives of Allergy & Immunology;2004, Vol. 135 Issue 2, p161 

    Background: Elevations of various cytokines, including Th1 and Th2 cytokines, have been reported in the acute phase of Kawasaki syndrome (KS). As interleukin (IL)-18 plays an important role in the Th1 cell response, investigating the relevance of IL-18 in KS should be helpful in determining the...

  • EIA.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p730 

    A definition of the acronym "EIA," which is enzyme immunoassay, is presented.

  • enzyme immunoassay.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p785 

    An encyclopedia entry for "enzyme immunoassay" is presented.

  • Oral Fluids Test Shows Great Potential. VanSickle, Joe // National Hog Farmer Expert Blog;1/20/2012, p2 

    The article discusses the first official swine oral fluid test for detecting antibodies against porcine reproductive and respiratory syndrome (PRRS) virus developed by Iowa State University (ISU) Veterinary Diagnostic Laboratory researchers. It cites the method used to interpret the test. The...

  • Validation of an ELISA developed for measuring cortisol concentration in human saliva and serum for its applicability to analyze cortisol in pig saliva. Thomsson, Ola; Ström-Holst, Bodil; Sjunnesson, Ylva; Bergqvist, Ann-Sofi // Acta Veterinaria Scandinavica;2014, Vol. 56 Issue 1, p1 

    Background The purpose of this study was to validate a commercially available enzyme-linked immunosorbent assay (ELISA) developed for measuring free cortisol in human saliva and total cortisol concentration in diluted human serum, for its applicability in measuring cortisol concentration in pig...

  • GALVIJŲ VIRUSINÄ–S DIARÄ–JOS PAPLITIMAS LIETUVOJE IR KONTROLÄ–S PRIEMONIŲ EFEKTYVUMAS NUSTATANT PERSISTENTIÅ KAI INFEKUOTUS GALVIJUS. Kliučinskas, Rolanas; Lukauskas, Kazimieras; Milius, Jonas; Mauricas, Mykolas // Veterinarija ir Zootechnika;2004, Vol. 28 Issue 50, p5 

    The aim of this study was to evaluate an effectiveness of control measures for bovine viral diarrhoea vires (BVDV) in Lithuania during 2001–2003. The BVD investigation data used in this article were supplied by the reference National Veterinary Laboratory (NVL). An enzym-linked...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics