TITLE

Assisted hatching using a 1.48-µm diode laser: Evaluation of zona opening and zona thinning techniques in human embryos

AUTHOR(S)
Yano, Kohji; Kubo, Toshiko; ÔHashi, Ikuko; Yano, Chieko
PUB. DATE
September 2006
SOURCE
Reproductive Medicine & Biology;Sep2006, Vol. 5 Issue 3, p221
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Aim: We assessed the effectiveness of assisted hatching using a 1.48-µm diode laser in human embryos, comparing zona opening and zona thinning techniques. Methods: A total of 56 day 3 embryos were assigned randomly to a zona opening group, a zona thinning group, or a control group. We then carried out assisted hatching using the OCTAX Laser Shot system (MTG Medical Technology, Altdorf, Germany) in the first two groups. In the zona opening group, the inner membrane of the zona pellucida was broken to create a full-thickness opening. In the zona thinning group, the inner membrane of the zona pellucida was not breached. After the laser procedure, embryos were cultured to the hatched blastocyst stage. Results: Blastocyst development rates did not differ significantly between the three groups. In the zona opening group, blastocysts were significantly more likely to hatch than those in the control group ( P ≤ 0.05) and no arrested hatching of blastocysts was observed. Conclusions: Assisted hatching using a 1.48-µm diode laser in the zona opening technique increases the likelihood of blastocyst hatching in human embryos and does not adversely effect subsequent embryo development. (Reprod Med Biol 2006; 5: 221–226)
ACCESSION #
21844337

 

Related Articles

  • Coculture with a Human Granulosa Cell Line Enhanced the Development of Murine Preimplantation Embryos Via SCF/c-Kit System. Taniguchi, Fuminori; Harada, Tasuku; Nara, Makoto; Deura, Imari; Mitsunari, Masahiro; Terakawa, Naoki // Journal of Assisted Reproduction & Genetics;Jun2004, Vol. 21 Issue 6, p223 

    Purpose: To evaluate the effect on the coculture of murine embryos with a human ovarian granulosa tumor derived cell line (KGN cells). Methods: We observed microscopically the growth of murine preimplantation embryos in the coculture system with KGN cells or in the presence with exogenous stem...

  • Session 26: Paramedical invited session – Laboratory: The embryo-endometrial interface – role in implantation/developmental success.  // Human Reproduction;Jun2013, Vol. 28 Issue suppl_1, pi40 

    No abstract available.

  • Number and Size of Antral Follicles as Predictive Factors in Vitro Fertilization and Embryo... Pohl, Michaela; Hohlagschwandtner, Maria // Journal of Assisted Reproduction & Genetics;Jul2000, Vol. 17 Issue 6, p315 

    Evaluates whether number and size of antral follicles can predict the outcome of in vitro-fertilization-embryo transfer. Use of three-dimensional transvaginal ultrasound technology; Application of gonadotropin; Implantation rate, number of retrieved oocytes and endometrium thickness.

  • Number and Size of Antral Follicles as Predictive Factors in Vitro Fertilization and Embryo... Pohl, Michaela; Hohlagschwandtner, Maria // Journal of Assisted Reproduction & Genetics;Jul2000, Vol. 17 Issue 6, p315 

    Evaluates whether number and size of antral follicles can predict the outcome of in vitro-fertilization-embryo transfer. Use of three-dimensional transvaginal ultrasound technology; Application of gonadotropin; Implantation rate, number of retrieved oocytes and endometrium thickness.

  • Eight years' experience with an IVF surrogate gestational pregnancy programme. Raziel, Arieh; Schachter, Morey; Strassburger, Deborah; Komarovsky, Dafna; Ron-El, Raphael; Friedler, Shevach // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Aug2005, Vol. 11 Issue 2, p254 

    The aim of this study was to retrospectively audit eight years' experience of an IVF surrogate gestational programme and to compare the outcome of surrogacy due lo absence of the uterus with surrogacy indicated for repeated IVF failure and recurrent abortions. A total of 60 cycles of IVF...

  • Parameters predicting the implantation rate of thawed IVF/ICSI embryos: a retrospective study. Gabrielsen, A.; Fedder, J.; Agerholm, I. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jan2006, Vol. 12 Issue 1, p70 

    Single embryo transfer is becoming increasingly popular in IVF/ICSI. More IVF/ICSI cycles therefore include freezing of high quality embryos, and the cumulative effect of such cycles becomes more important. To improve the results obtained using frozen thawed embryos, the predictive value of...

  • FSH receptor genotype is associated with pregnancy but not with ovarian response in IVF. Klinkert, Ellen R.; Te Velde, Egbert R.; Weima, Sjerp; Van Zandvoort, Peter M.; Hanssen, Rob G. J. M.; Nilsson, Philomeen R.; De Jong, Frank H.; Looman, Caspar W. N.; Broekmans, Frank J. M. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Nov2006, Vol. 13 Issue 5, p687 

    Two very common single nucleotide polymorphisms at positions 307 and 680 in exon 10 of the FSH receptor gene have been associated with ovarian response in IVF. This observational study evaluated the role of the FSH receptor genotype in the prediction of poor response and clinical pregnancy in...

  • Embryo implantation: the molecular mechanism remains elusive. Aplin, J. D. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Dec2006, Vol. 13 Issue 6, p833 

    Low rates of implantation are an impediment to more efficient assisted reproduction techniques. Improved endometrial receptivity and embryo preparation should lead to higher pregnancy rates, lower rates of early pregnancy failure and fewer multiple pregnancies. As the first site of contact...

  • Human implantation: the last barrier in assisted reproduction technologies? Edwards, Robert G. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Dec2006, Vol. 13 Issue 6, p887 

    Implantation processes are highly complex involving the actions of numerous hormones, immunoglobulins, cytokines and other factors in the endometrium. They are also essential matters for the success of assisted reproduction. The nature of early embryonic development is of equal significance. It...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics