TITLE

Preparation of Pooled Arabidopsis YAC DNAs for PCR-Based Mapping

AUTHOR(S)
Naested, Henrik; Jensen, Anders Bogh
PUB. DATE
March 1999
SOURCE
Plant Molecular Biology Reporter;1999, Vol. 17 Issue 1, p67
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract. Physical mapping of an arabidopsis DNA sequence of interest can easily be performed by PCR. This is done by using specific primers and pooled DNA templates isolated from publicly available YAC or BAC libraries. We present simple protocols for preparing pooled YAC DNAs and PCR-based detection of sequences in them by which several sequences can be mapped in a short time.
ACCESSION #
2513019

 

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