TITLE

Involvement of the Mitochondrial Protein Translocator Component Tim50 in Growth, Cell Proliferation and the Modulation of Respiration in Drosophila

AUTHOR(S)
Sugiyama, Shin; Moritoh, Satoru; Furukawa, Yoshimi; Mizuno, Tomohiko; Young-Mi Lim; Tsuda, Leo; Nishida, Yasuyoshi
PUB. DATE
June 2007
SOURCE
Genetics;Jun2007, Vol. 176 Issue 2, p927
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Allelic mutants exhibiting growth defects in Drosophila were isolated. Molecular cloning identified the responsible gene as a budding yeast Tim50 ortholog, and thus it was named tiny tim 50 (ttm50). The weak allele (ttm50Gp99) produced small flies due to reduced cell size and number, and growth terminated at the larval stage in the strong alleles (ttm50IE1 and ttm50IE2). Twin-spot analysis showed fewer cells in ttm50Gp99 clones, whereas ttm50IE1 clones did not proliferate, suggesting that the gene has an essential cellular function. Tim50 is known to maintain mitochondrial membrane potential (MMP) while facilitating inner- membrane protein transport. We found that tagged Ttm50 also localized to mitochondria and that mitochondrial morphology and MMP were affected in mutants, indicating that mitochondrial dysfunction causes the developmental phenotype. Conversely, ttm50 overexpression increased MMP and apoptosis. Co-expression of p35 suppressed this apoptosis, resulting in cell overproliferation. Interestingly, ttm50 transcription was tissue specific, corresponding to elevated MMP in the larval midgut, which was decreased in the mutant. The correlation of ttm50 expression levels with differences in MMP match its proposed role in mitochondrial permeability barrier maintenance. Thus a mitochondrial protein translocase component can play active roles in regulating metabolic levels, possibly for modulation of physiological function or growth in development.
ACCESSION #
25790360

 

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