TITLE

Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosis of schistosomiasis mansoni

AUTHOR(S)
de Oliveira, Edward José; Kanamura, Hermínia Yohko; Takei, Kioko; Hirata, Rosario Dominguez Crespo; Valli, Luiz Carlos Pedrosa; Nguyen, Nga Yen; de Carvalho Rodrigues, Izabel; de Jesus, Amélia Ribeiro; Hirata, Mário Hiroyuki
PUB. DATE
April 2008
SOURCE
Transactions of the Royal Society of Tropical Medicine & Hygiene;Apr2008, Vol. 102 Issue 4, p360
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Summary: A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of Schistosoma mansoni. Serum samples were obtained from individuals with acute (n =23) and chronic (n =30) schistosomiasis, with other parasitoses (n =39) or without parasitic infections (n =100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (IgM-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8% and 94.2% when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in κ concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher levels in patients with acute compared with chronic schistosomiasis (P = 0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni.
ACCESSION #
31306381

 

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