TITLE

Boophilus microplus Pyrethroid Resistance Associated to Increases Levels of Monooxygenase Enzymatic Activity in Field Isolated Mexican Ticks

AUTHOR(S)
Cossío-Bayúgar, Raquel; Miranda-Miranda, Estefan; Ortiz-Nájera, Alejandrina; Neri-Orantes, Salvador
PUB. DATE
April 2008
SOURCE
Journal of Biological Sciences;2008, Vol. 8 Issue 2, p404
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This study was made to assess SDS-PAGE monooxygenases zymograms in the presence of synthetic monooxygenase enzyme substrates, in order to establish a reliable methodology to measure monooxygenase activity levels on acaricide resistant ticks and finding a probable association between monooxygenase enzymatic levels and B. microplus acaricide resistance. Monooxygenase activity was found in the zymograms of the analyzed ticks at three polypeptidic bands of 147, 125 and 98 kDa, densitometric measurements of monooxygenases zymograms at these specific enzymatic bands showed a statistically significant monooxygenase activity increase (p<0.001 unpaired t-test) on Pyrethroid resistant ticks when compared to the same bands in the acaricide susceptible reference ticks. Present results showed that monooxygenase zymograms combined with densitographic analysis, are useful as an acaricide resistance detection test and suggest a linkage between increased monooxygenase activities and Pyrethroid acaricide resistance in B. microplus.
ACCESSION #
33007650

 

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