TITLE

Evaluation of the mineralizable organic nitrogen in soil using an immunological technique

AUTHOR(S)
Shingo Matsumoto; Noriharu Ae; Yohichi Koyama; Katsumasa Iijima; Rumiko Kodashima; Mari Hirata; Junko Kasuga; Nobuhisa Koga
PUB. DATE
October 2008
SOURCE
Biology & Fertility of Soils;Oct2008, Vol. 45 Issue 1, p107
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract  Phosphate buffer-extractable organic nitrogen (PEON), which can be extracted from soil with a 67-mM neutral phosphate buffer solution, is considered to be a major source of mineralizable nitrogen (N) that is ubiquitous in soil. We have developed a Western blot analysis and enzyme-linked immune sorbent assay (ELISA) method for determining PEON. First, anti-PEON antibody (IgG) from a rabbit was produced using purified PEON from a volcanic ash soil. PEON was detected as a ladder-like staining pattern in the high-molecular-weight region and as a single band under 14 kDa in Western blot analysis using the anti-PEON IgG. In the ELISA assay, the anti-PEON IgG could specifically react with PEON irrespective of soil type and management. Furthermore, the absorbance at 450 nm in ELISA was highly correlated to the concentration of organic N (Norg) in phosphate extracts of different soils. We propose the immune assay using the anti-PEON IgG as a possible novel method for evaluating available N in soil.
ACCESSION #
34573647

 

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