TITLE

Excision of a selective marker in transgenic rice using a novel Cre/ loxP system controlled by a floral specific promoter

AUTHOR(S)
Chengcai Chu
PUB. DATE
December 2008
SOURCE
Transgenic Research;Dec2008, Vol. 17 Issue 6, p1035
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract  Based on the Cre/loxP system, we have developed a novel marker-free system mediating a direct auto-excision of loxP-flanked marker genes from T1 transgenic rice without any treatment or further offspring crossing. To achieve this, the floral-specific promoter OsMADS45 was isolated from rice and the expression patterns of OsMADS45 promoter was characterised by using the pOs45:GUS transgenic plants. Furthermore, the binary vector with Cre recombinase under the control of OsMADS45 promoter was constructed and introduced into rice by Agrobacterium-mediated transformation and transgenic rice plants were generated. Southern blot analysis showed that auto-excision of the selective markers occurred in some T1 progeny of the transgenic plants, suggesting that a high auto-excision frequency can be achieved with our Cre/loxP system. This auto-excision strategy provides an efficient way of removing the selectable marker gene from transgenic rice.
ACCESSION #
34935773

 

Related Articles

  • A question of breeding.  // New Scientist;3/19/2005, Vol. 185 Issue 2491, p5 

    The article focuses on scepticism of consumers regarding new genetically modified (GM) crop. One of the biggest hurdles in selling genetically modified crops to sceptical consumers, especially in Europe, has been that there was nothing in it for them. All the traits commercialised so far, such...

  • Identification and functional characterization of a rice NAC gene involved in the regulation of leaf senescence. Yong Zhou; Weifeng Huang; Li Liu; Taiyu Chen; Fei Zhou; Yongjun Lin // BMC Plant Biology;2013, Vol. 13 Issue 1, p1 

    Background: As the final stage of leaf development, leaf senescence may cause the decline of photosynthesis and gradual reduction of carbon assimilation, which makes it a possible limiting factor for crop yield. NACs are plantspecific transcription factors and some NACs have been confirmed to...

  • Genetically Modified Foods and Seeds. Hanly, Ken // Canadian Dimension;Mar/Apr2000, Vol. 34 Issue 2, p12 

    Discusses the aspects of biotechnology and suggests several means for its socialization. Adoption of policies within capitalism to minimize risks; Environmental risks of genetically modified (GM) foods and seeds; Criticism that GM seeds may increase the use of pesticide.

  • Inhibition of chloroplastic fructose 1,6-bisphosphatase in tomato fruits leads to decreased fruit size, but only small changes in carbohydrate metabolism. Hazem Obiadalla-Ali; Alisdair R. Fernie; Anna Lytovchenko; Jens Kossmann; James R. Lloyd // Planta;Jul2004, Vol. 219 Issue 3, p533 

    A potato ( Solanum tuberosum L. ) cDNA coding for the chloroplastic isoform of fructose 1,6-bisphosphatase (cp-FBPase) was utilized to repress its activity in tomatoes ( Lycopersicon esculentum Mill.) using antisense techniques. The patatin B33 promoter was used to ensure fruit specificity of...

  • Introduction to ISBGMO12: biosafety research past, present and future. Raybould, Alan; Quemada, Hector; Romeis, Jörg // Transgenic Research;Dec2014, Vol. 23 Issue 6, p911 

    An introduction is presented in which the editor discusses various reports published within the issue which include genetically modified (GM) crops, the molecular analysis of genetic changes in GMs, and the concerns about hte effects of weedy or invasive GM crops.

  • Visual selection allows immediate identification of transgenic rice calli efficiently accumulating transgene products. Hiroaki Saika; Seiichi Toki // Plant Cell Reports;Apr2009, Vol. 28 Issue 4, p619 

    Abstract  In genetic transformation systems, antibiotic resistance genes are routinely used as powerful markers for selecting transformed cells from surrounding non-transformed cells. However, simultaneous use of the gene encoding green fluorescent protein (GFP) and an antibiotic...

  • Mutations in the rice liguleless gene result in a complete loss of the auricle, ligule, and laminar joint. Jinwon Lee; Jong-Jin Park; Song Kim; Jieun Yim; Gynheung An // Plant Molecular Biology;Nov2007, Vol. 65 Issue 4, p487 

    Abstract  The area between the upper part of the leaf sheath and the basal portion of the leaf blade contains several specialized organs, such as the laminar joint, auricle and ligule. Here we report the identification of T-DNA insertional mutant lines that lack all of these organs. The...

  • The promoter of a rice glycine-rich protein gene, Osgrp-2, confers vascular-specific expression in transgenic plants. Zong-Zhi Liu; Jian-Long Wang; Xun Huang; Wei-Hui Xu; Zhao-Ming Liu; Rong-Xiang Fang // Planta;Mar2003, Vol. 216 Issue 5, p824 

    The genomic sequence of a rice (Oryza sativa L.) glycine-rich protein (GRP) gene, designated Osgrp-2, has been previously determined (GenBank U40708). Primer extension analysis indicated that transcription starts 47 bp upstream of the translation start codon. To gain an insight into the...

  • Functional analysis of rice HOMEOBOX4 ( Oshox4 ) gene reveals a negative function in gibberellin responses. Mingqiu Dai; Yongfeng Hu; Qian Ma; Yu Zhao; Dao-Xiu Zhou // Plant Molecular Biology;Feb2008, Vol. 66 Issue 3, p289 

    Abstract  The homeodomain-leucine zipper (HD-Zip) putative transcription factor genes are divided into 4 families. In this work, we studied the function of a rice HD-Zip I gene, H OME O BO X4 (Oshox4). Oshox4 transcripts were detected in leaf and floral organ primordia but...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics