TITLE

Development of a Toxicity Identification Evaluation Protocol Using Chlorophyll- a Fluorescence in a Marine Microalga

AUTHOR(S)
Strom, D.; Ralph, P.; Stauber, J.
PUB. DATE
January 2009
SOURCE
Archives of Environmental Contamination & Toxicology;Jan2009, Vol. 56 Issue 1, p30
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Growth inhibition bioassays with the microalga Nitzschia closterium have recently been applied in marine Toxicity Identification Evaluation (TIE) testing. However, the 48-h test duration can result in substantial loss of toxicants over time, which might lead to an underestimation of the sample toxicity. Although shorter-term microalgal bioassays can minimize such losses, there are few bioassays available and none are adapted for marine TIE testing. The acute (5-min) chlorophyll- a fluorescence bioassay is one alternative; however, this bioassay was developed for detecting herbicides in freshwater aquatic systems and its suitability for marine TIE testing was not known. In this study, a chlorophyll- a fluorescence bioassay using the marine microalga Isochrysis galbana was able to detect contaminants other than herbicides at environmentally relevant concentrations and tolerated the physical and chemical manipulations needed for a Phase I TIE. Phase I TIE procedures were successfully developed using this chlorophyll- a fluorescence bioassay and used to identify all classes of contaminants present in a synthetic mixture of known chemical composition. In addition, TIEs with both the acute fluorescence bioassay and the standard growth inhibition bioassay identified the same classes of toxicants in a sample of an unknown complex effluent. Even though the acute chlorophyll- a fluorescence end point was less sensitive than the chronic cell division end point, TIEs with the chlorophyll- a fluorescence bioassay provided a rapid and attractive alternative to longer-duration bioassays.
ACCESSION #
35774188

 

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