TITLE

Matrix alkalinization: a novel mitochondrial signal for sustained pancreatic β-cell activation

AUTHOR(S)
Wiederkehr, Andreas; Kyu-Sang Park; Dupont, Olivier; Demaurex, Nicolas; Pozzan, Tullio; Cline, Gary W.; Wollheim, Claes B.
PUB. DATE
February 2009
SOURCE
EMBO Journal;2/25/2009, Vol. 28 Issue 4, p417
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Nutrient secretagogues activate mitochondria of the pancreatic β-cell through the provision of substrate, hyperpolarisation of the inner mitochondrial membrane and mitochondrial calcium rises. We report that mitochondrial matrix pH, a parameter not previously studied in the β-cell, also exerts an important control function in mitochondrial metabolism. During nutrient stimulation matrix pH alkalinises, monitored by the mitochondrial targeted fluorescent pH-sensitive protein mtAlpHi or 31P-NMR inorganic phosphate chemical shifts following saturation transfer. Compared with other cell types, the resting mitochondrial pH was surprisingly low, rising from pH 7.25 to 7.7 during nutrient stimulation of rat β-cells. As cytosolic alkalinisation to the nutrient was of much smaller amplitude, the matrix alkalinisation was accompanied by a pronounced increase of the ΔpH across the inner mitochondrial membrane. Furthermore, matrix alkalinisation closely correlates with the cytosolic ATP net increase, which is also associated with elevated ATP synthesis rates in mitochondria. Preventing ΔpH increases in permeabilised cells abrogated substrate-driven ATP synthesis. We propose that the mitochondrial pH and ΔpH are key determinants of mitochondrial energy metabolism and metabolite transport important for cell activation.
ACCESSION #
36555361

 

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