TITLE

Visual selection allows immediate identification of transgenic rice calli efficiently accumulating transgene products

AUTHOR(S)
Hiroaki Saika; Seiichi Toki
PUB. DATE
April 2009
SOURCE
Plant Cell Reports;Apr2009, Vol. 28 Issue 4, p619
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract  In genetic transformation systems, antibiotic resistance genes are routinely used as powerful markers for selecting transformed cells from surrounding non-transformed cells. However, simultaneous use of the gene encoding green fluorescent protein (GFP) and an antibiotic resistance gene facilitates the selection process, since it allows visible selection of transformed cells. Here, we report the development of a visual selection system for transformed cells using a GFP marker without selection against antibiotics after Agrobacterium-mediated transformation in rice. Both GFP protein levels and GFP fluorescence in calli isolated by visual selection were higher than in calli selected on hygromycin (Hyg), suggesting that transgenic calli hyper-accumulating GFP were efficiently obtained by selection using GFP fluorescence itself rather than Hyg resistance. Furthermore, gfp transcripts in calli isolated by visual selection were more abundant than under Hyg selection; in contrast, transcript levels of hpt in calli selected visually were comparable to those obtained under Hyg selection. These results suggest that there was no correlation between hpt and gfp expression levels, despite the fact that they are aligned in tandem on an integrated locus after selection by either GFP fluorescence or Hyg resistance. This fact indicates that positional effects can influence the expression of each transgene differently, even when they are located in tandem at the same locus. In summary, based on our results, we discuss a model system for rice cell culture transformation for the production of recombinant proteins using visual selection.
ACCESSION #
37228436

 

Related Articles

  • Excision of a selective marker in transgenic rice using a novel Cre/ loxP system controlled by a floral specific promoter. Chengcai Chu // Transgenic Research;Dec2008, Vol. 17 Issue 6, p1035 

    Abstract  Based on the Cre/loxP system, we have developed a novel marker-free system mediating a direct auto-excision of loxP-flanked marker genes from T1 transgenic rice without any treatment or further offspring crossing. To achieve this, the floral-specific promoter OsMADS45...

  • A question of breeding.  // New Scientist;3/19/2005, Vol. 185 Issue 2491, p5 

    The article focuses on scepticism of consumers regarding new genetically modified (GM) crop. One of the biggest hurdles in selling genetically modified crops to sceptical consumers, especially in Europe, has been that there was nothing in it for them. All the traits commercialised so far, such...

  • Improved Salinity Tolerance of Rice Through Cell Type- Specific Expression of AtHKT1;1. Plett, Darren; Safwat, Gehan; Gilliham, Matthew; Møller, Inge Skrumsager; Roy, Stuart; Shirley, Neil; Jacobs, Andrew; Johnson, Alexander; Tester, Mark // PLoS ONE;2010, Vol. 5 Issue 9, p1 

    Previously, cell type-specific expression of AtHKT1;1, a sodium transporter, improved sodium (Na+) exclusion and salinity tolerance in Arabidopsis. In the current work, AtHKT1;1, was expressed specifically in the root cortical and epidermal cells of an Arabidopsis GAL4-GFP enhancer trap line....

  • Overexpression of a high-affinity phosphate transporter gene from tobacco ( NtPT1) enhances phosphate uptake and accumulation in transgenic rice plants. Park, Myoung; Baek, So-Hyeon; Reyes, Benildo; Yun, Song // Plant & Soil;Mar2007, Vol. 292 Issue 1/2, p259 

    High-affinity phosphate transporters (PTs) have been suggested as major components of mechanisms of inorganic phosphate (Pi) uptake in plant cells. In this study, we investigated the role of PT in improving Pi uptake by transgenic overexpression of a tobacco ( Nicotiana tabacum L.) gene encoding...

  • Identification and functional characterization of a rice NAC gene involved in the regulation of leaf senescence. Yong Zhou; Weifeng Huang; Li Liu; Taiyu Chen; Fei Zhou; Yongjun Lin // BMC Plant Biology;2013, Vol. 13 Issue 1, p1 

    Background: As the final stage of leaf development, leaf senescence may cause the decline of photosynthesis and gradual reduction of carbon assimilation, which makes it a possible limiting factor for crop yield. NACs are plantspecific transcription factors and some NACs have been confirmed to...

  • Effect of Transgenic Bt Rice on the Survival of Three Nontarget Stored Product Insect Pests. Riudavets, Jordi; Gabarra, Rosa; Pons, M. José; Messeguer, Joaquima // Environmental Entomology;Oct2006, Vol. 35 Issue 5, p1432 

    The effects of Bacillus thuvingiensis (Bt) transgenic Ariete and Senia rice lines developed for the control of Chilo suppressalis Walker were evaluated on three nontarget stored product insect pests: the lepidopteran Plodia interpunctella (Hübner),the coleopteran Sitophilus oryzae L., and the...

  • PREVENTATIVE V. PUNITIVE: HOW GENETICALLY MODIFIED RICE LITIGATION SHAPED REGULATION AND REMEDY FOR GENETICALLY ENGINEERED CROPS. Bragg, Allison Waldrip // Journal of Food Law & Policy;Fall2014, Vol. 10 Issue 2, p157 

    The article explores events that led to the litigation and regulations to prevent introduction of regulated genetically engineered product into non-engineered supply and remedies available. It considers regulated articles as organisms altered or created by genetic engineering and can be regarded...

  • The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice. Takasaki, Hironori; Maruyama, Kyonoshin; Kidokoro, Satoshi; Ito, Yusuke; Fujita, Yasunari; Shinozaki, Kazuo; Yamaguchi-Shinozaki, Kazuko; Nakashima, Kazuo // Molecular Genetics & Genomics;Sep2010, Vol. 284 Issue 3, p173 

    The transcription factor OsNAC5 in rice is a member of the plant-specific NAC family that regulates stress responses. Expression of OsNAC5 is induced by abiotic stresses such as drought, cold, high salinity, abscisic acid and methyl jasmonic acid. Transactivation assays using rice protoplasts...

  • Can genetically modified organisms feed the world? Demenet, Philippe // UNESCO Courier;Sep2001, Vol. 54 Issue 9, p10 

    Investigates the effectiveness of genetically modified organisms (GMO) as a guarantee for food security of the world's undernourished individuals. Real reason for the malnutrition of the poor; Information on the development of revolutionary rice Nerica in Africa; Differences between Nerica and...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics