TITLE

Isolation of Genomic DNA from Defatted Oil Seed Residue of Opium Poppy (Papaver sominiferum)

AUTHOR(S)
Sangwan, R.S.; Yadav, U.; Sangwan, N.S.
PUB. DATE
September 2000
SOURCE
Plant Molecular Biology Reporter;2000, Vol. 18 Issue 3, p265
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract. A simple protocol for obtaining pure, restrictable and amplifiable megabase genomic DNA from oil free seed residue of Papaver sominiferum, an important oil seed and morphanian alkaloid yielding plant, has been developed. Fixed oil from the dry seeds was completely recovered in an organic solvent and quantified gravimetrically followed by processing of the residual biomass (defatted seed residue) for genomic DNA isolation. The isolated DNA could be cut by a range of restriction enzymes. The method enables simultaneous isolation and recovery of lipids and genomic DNA from the same test sample, thus allowing two independent analyses from a single sample.
ACCESSION #
3861893

 

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