TITLE

A protein microarray immunoassay for the serological evaluation of the antibody response in vertically transmitted infections

AUTHOR(S)
Ardizzoni, A.; Capuccini, B.; Baschieri, M.; Orsi, C.; Rumpianesi, F.; Peppoloni, S.; Cermelli, C.; Meacci, M.; Crisanti, A.; Steensgaard, P.; Blasi, E.
PUB. DATE
September 2009
SOURCE
European Journal of Clinical Microbiology & Infectious Diseases;Sep2009, Vol. 28 Issue 9, p1067
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The detection of specific serum antibodies is mainly achieved by enzyme-linked immunosorbent assay (ELISA). Here, we describe the setting up of a microarray-based serological assay to screen for IgG and IgM against vertically transmitted pathogens ( Toxoplasma gondii, rubella virus, cytomegalovirus, herpes simplex virus types 1 and 2, varicella zoster virus, Chlamydia trachomatis). The test, accommodated onto a restricted area of a microscope slide, consists of: (a) the immobilization of antigens and human IgG and IgM antibody dilution curves, laid down in an orderly manner; (b) addition of serum samples; (c) detection of antigen–serum antibodies complexes by indirect immunofluorescence. The IgG and IgM curves provide an internal calibration system for the interpolation of the signals from the single antigens. The test was optimized in terms of spotting conditions and processing protocol. The detection limit was 400 fg for the IgG assay and 40 fg for the IgM assay; the analytical specificity was >98%. The clinical sensitivity returned an average value of 78%, the clinical specificity was >96%, the predictive values were >73%, and the efficiency was >88%. The results obtained make this test a promising tool, suitable for introduction in the clinical diagnostic routine of vertically transmitted infections, in parallel (and in future as an alternative) to ELISA.
ACCESSION #
44411871

 

Related Articles

  • Study the Anti-Toxoplasma Antibodies (IgG and IgM) in Hemodialysis Patients of Abadan and Khoramshahr Cities Southwest Iran in 2011 using ELISA. Maraghi, Sharif; Yadyad, Mohammad Jafar; Sheikhi, Marjan; Shamakhteh, Fatemeh; Latifi, Seyed Mahmood // Jundishapur Journal of Microbiology;Sep2013, Vol. 6 Issue 7, p1 

    Background: Toxoplasmosis is a worldwide disease caused by an obligate intracellular protozoan, Toxoplasma gondii and can cause severe infections in immune- compromised individuals. Objectives: The aim of this study was to determinate the anti- Toxoplasma antibodies in hemodialysis patients of...

  • Prevalence and Risk Factors of Toxoplasma gondii Infection in Domestic Cats from the Tropics of Mexico Using Serological and Molecular Tests. Castillo-Morales, Virgen J.; Acosta Viana, Karla Y.; Guzmán-Marín, Eugenia del S.; Jiménez-Coello, Matilde; Segura-Correa, José C.; Aguilar-Caballero, A. J.; Ortega-Pacheco, Antonio // Interdisciplinary Perspectives on Infectious Diseases;2012, p1 

    The aim of this study was to determine the prevalence and risk factors associated with Toxoplasma gondii infection in domestic cats using an indirect-ELISA (IgM and IgG) and PCR. Samples collected from 220 cats from Merida, Yucatan, Mexico, were analyzed. Cases were reported as acute or chronic....

  • Detection of toxoplasma antibodies in sera of Salmonidae by ELISA. Taghadosi, Camellia; Kojouri, Gholam Ali; Taheri, Maryam Akhavan // Comparative Clinical Pathology;Apr2010, Vol. 19 Issue 2, p203 

    Toxoplasma gondii is a protozoan parasite with a worldwide distribution. It is capable of infecting all warmblooded animals. Toxoplasmosis was not considered a waterborne zoonoses, but recently, it has been reported in many marine mammals. Coastal pollution by sewage from humans and pets has...

  • IGM and IGG Response to 29-35-kDa Toxoplasma gondii Protein Fractions in Experimentally Infected Goats. de Felipe, M. M. Conde; Molina, J. M.; Rodriguez-Ponce, E.; Ruiz, A.; Gonzalez, J. F. // Journal of Parasitology;Jun2007, Vol. 93 Issue 3, p701 

    The article presents a study to determine if Toxoplasma gondii fractions recognized by immunoglobulin G (IgG) in infected goats could be useful in the detection of specific immunoglobulin M (IgM) and to evaluate the development of both isotypes throughout the infection. Results showed that the...

  • Toxoplasma gondii, Sitomegalovirus ve Rubella Infeksiyonlarının Tanısında Avidite Testleri: Ocak 2008-Aralık 2009 Tarihleri Arasındaki Sonuçların DeÄŸerlendirilmesi. Ziver, Tevhide; Yüksel, Pelin; Aslan, Mustafa; Sarıbaş, Suat; İzmirli, Sena; Güngördü, Zeynep; Kocazeybek, Bekir // Klimik Journal / Klimik Dergisi;Dec2010, Vol. 23 Issue 3, p105 

    Objective: Between January 2008 and December 2009 the Microbiology and Clinical Microbiology Department, Serology/ ELISA Laboratory evaluated the detection of three infectious agents and we aimed to evaluate the algorithmic position of avidity index (AI) test results in the clinician-laboratory...

  • Development of 116 kDa Fraction for Detecting Experimental Toxoplasma gondii Infections in Mice. Abdel-Hafez HASSANAIN, Mohey; Hussien ABDEL-RAHMAN, Eman; Ibrahim TOALEB, Nagwa; Mohamed SHAAPAN, Raafat; Ali ELFADALY, Hasan; Abdel-Hafez HASSANAIN, Nawal // Iranian Journal of Parasitology;2013, Vol. 8 Issue 3, p441 

    Background: Serological diagnosis of Toxoplasma gondii infection using crude antigens may not be more accurate. To increase the diagnostic potency of antigens, isolation of their immunogenic fractions could be useful. The current research adopted to obtain an affinity isolated fraction from RH...

  • IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans. Rahbari, Amir Hossien; Keshavarz, Hossien; Shojaee, Saeedeh; Mohebali, Mehdi; Rezaeian, Mostafa // Korean Journal of Parasitology;Jun2012, Vol. 50 Issue 2, p99 

    Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test...

  • Seroprevalence of Toxoplasma gondii Infection in Female Sterility Patients in China. Shuhong Li; Liming Cui; Jixue Zhao; Pei Dai; Shan Zong; Wenjing Zuo; Chen Chen; Hongtao Jin; Hongwei Gao; Quan Liu // Journal of Parasitology;Jun2011, Vol. 97 Issue 3, p529 

    Toxoplasmosis is an important parasitic disease worldwide and is related to certain psychiatric disorders and sterility. In the present study, serum samples from 882 female sterility patients and 107 pregnant- puerperant women were assayed for anti-T, gondii IgG antibodies using ELISA. The...

  • Gebelerde toxoplasma gondii seropozitifliÄŸinin ve IgG-Avidite sonuçlarının deÄŸerlendirilmesi. Ergün, Ayşe Gül; Öztürk, Tuba; Çiftçi, Esra; Aynali, Ayşe; Önal, Süleyman; Kaya, Selçuk // SDU Journal of Health Science Institute / SDÃœ Saglik Bilimleri ;2013, Vol. 4 Issue 3, p91 

    Objective: Toxoplasma gondii is a mandatory intracellular parasite. Generally asymptomatic, but it can lead to lifethreatening infections at congenitally infected newborns and immunocompromised patients. In this study, we aimed to identify the T. gondii seropositivity at pregnant women and to...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics