TITLE

Biodegradation of thiocyanate using co-culture of Klebsiella pneumoniae and Ralstonia sp

AUTHOR(S)
Chaudhari, Ashvini U.; Kodam, Kisan M.
PUB. DATE
January 2010
SOURCE
Applied Microbiology & Biotechnology;Jan2010, Vol. 85 Issue 4, p1167
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Thiocyanate-degrading microbial co-culture was isolated from thiocyanate-contaminated site and tested for thiocyanate degradation potential and thiocyanate-toxicity tolerance and identified as Klebsiella pneumoniae and Ralstonia sp. by 16S rDNA sequencing. The co-culture was able to degrade thiocyanate with degradation rate of 500 mg L−1d−1 at 2,500 mg L−1 thiocyanate concentration at pH 6.0 and 37ºC following thiocyanate hydrolase pathway. The Haldane kinetic model elucidates the growth and thiocyanate biodegradation kinetics of the co-culture with Ki value of 1,876 mg L−1. The thiocyanate biodegradation kinetics was not affected by the additional supply of glucose. The very high activities of thiocyanate hydrolase, cyanide oxygenase, and cytochrome P-450 content during growth on thiocyanate were observed, showing the induction mechanism.
ACCESSION #
47437625

 

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