TITLE

Herramientas no invasivas en Venezuela: comparación entre las pruebas inmunoserológicas DAT, rK26 y rK39 en el diagnóstico de leishmaniasis visceral

AUTHOR(S)
Terán-Ángel, Guillermo; Rodríguez, Vestalia; Silva, Rosilved; Zerpa, Olga; Schallig, Henk; Ulrich, Marian; Cabrera, Maira
PUB. DATE
March 2010
SOURCE
Biomédica: Revista del Instituto Nacional de Salud;mar2010, Vol. 30 Issue 1, p39
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Introduction. Human visceral leishmaniasis is a serious public health problem in endemic countries because of its high potential lethality, particularly in children. Rapid diagnosis is essential to early treatment and control of visceral leishmaniasis. Objective. The aim was to compare three serodiagnostic tools for human visceral leishmaniasis. Materials and methods. Three methods were compared: the rK39 dipstick (Kalazar detection test, Inbios International Inc.), ELISA rK26 and direct agglutination test (DAT) (KIT Biomedical Research). Fifty serum samples from patients positive for rK39 ELISA were compared from four endemic provinces in Venezuela: Nueva Esparta (Margarita island), Lara, Anzoátegui and Trujillo. Additional serum samples from 17 healthy volunteers and 25 patients with other diseases were included. The rK39 ELISA served as the baseline standard method. Sensitivity, specificity, positive predictive value, negative predictive value and likelihood ratio were calculated for each test. Results. All methods had a positive correlation with rK39 ELISA (p<0.0001). They showed high sensitivity and specificity. The direct agglutination test and the rK39 dipstick showed high sensitivity values, 89.7% (95% CI: 81.34.0-98.2%) and 94.2% (95% CI: 87.7-100%), respectively, and high specificity, 81.0% (95% CI: 80.0-99.5%) and 100%. The rK26 ELISA showed good specificity, 99% (95% CI: 95.2-100%), but a very low sensitivity, 37% (95% CI: 23.4-50.2%). Conclusion. Overall results indicated that DAT and rK39 dipstick have the highest specificity and sensitivity. Both are simple, cost-effective and field applicable tests. Therefore, they are recommended for early and accurate diagnosis of visceral leishmaniasis.
ACCESSION #
49074402

 

Related Articles

  • Evaluation of Serological Tests for the Diagnosis of Visceral Leishmaniasis. Kiliç, Selçuk; Taylan Özkan, Ayşegül; Babür, Cahit; Tanir, Gönül; Dfh Schallig, Henk // Turkish Journal of Medical Sciences;2008, Vol. 38 Issue 1, p13 

    Aim: Visceral leishmaniasis (VL) is a zoonosis that affects both animals and man. VL is seen sporadically in the Aegean, Mediterranean, Black Sea and central Anatolian regions of Turkey. The aim of this study was to evaluate serological tests for the diagnosis of VL. Materials and Methods: The...

  • Evaluation of the one-point microcapsule agglutination test for diagnosis of leptospirosis. Arimitsu, Y.; Kmety, E.; Ananyina, Y.; Baranton, G.; Ferguson, I. R.; Smythe, L.; Terpstra, W. J. // Bulletin of the World Health Organization;1994, Vol. 72 Issue 3, p395 

    We have developed a one-point microcapsule agglutination test (MCA T) for the serodiagnosis of leptospirosis. The MCAT kit was evaluated for use in humans by six WHO Collaborating Centres for Reference and Research on Leptospirosis. The laboratories classified their serum samples on the basis of...

  • LABORATORY DIAGNOSIS AND CLINICAL SIGNS OF CANINE VISCERAL LEISHMANIASIS IN DOGS EXAMINED AT THE CENTER FOR ZOONOSIS CONTROL IN CAMPO GRANDE - MS, BRAZIL. Cunha, Rodrigo Casquero; Andreotti, Renato; Araujo e Silva, Elaine; Pereira, Elisângela; Sato, Tayra; Thomaz-Soccol, Vanete // Archives of Veterinary Science;2012, Vol. 17 Issue 4, p17 

    Visceral leishmaniasis is a type of zoonosis caused by several Leishmania species endemic to tropical, subtropical, and Mediterranean climate regions. Dogs are the primary source of infection in urban areas and can be symptomatic or asymptomatic. This study focused on the observation of clinical...

  • Evaluation of conventional & serological methods for rapid diagnosis of cryptococcosis. Saha, D. C.; Xess, Immaculata; Jain, Neena // Indian Journal of Medical Research;May2008, Vol. 127 Issue 5, p483 

    Background & objectives: Cryptococcosis is a chronic infective condition affecting the central nervous system. Unless diagnosed early and specific treatment instituted it can be fatal. There is an urgent need for a rapid and specific diagnostic tool for better management of the patients....

  • Approaches for the Development of Rapid Serological Assays for Surveillance and Diagnosis of Infections Caused by Zoonotic Flaviviruses of the Japanese Encephalitis Virus Serocomplex. Hobson-Peters, Jody // Journal of Biomedicine & Biotechnology;2012, Vol. 2012, p1 

    Flaviviruses are responsible for a number of important mosquito-borne diseases of man and animals globally. The short vireamic period in infected hosts means that serological assays are often the diagnostic method of choice. This paper will focus on the traditional methods to diagnose flaviviral...

  • Evaluation of tests for serological diagnosis of scrub typhus. Prakash, J. A. J.; Abraham, O. C.; Mathai, Elizabeth // Tropical Doctor;Oct2006, Vol. 36 Issue 4, p212 

    Two specific serological tests, a Dot enzyme immunoassay (EIA) and an immunoglobulin (Ig)M enzyme-linked immunosorbent assay (ELISA) using the 56 kDa antigen and the Weil–Felix test were evaluated for diagnosis of scrub typhus. Sensitivity of 100, 86.5 and 43.5% were observed with Dot...

  • Evaluation of a Latex Agglutination Test for Detection of Immunoglobulin M (IgM) and IgG Antibodies to Mycoplasma pneumoniae. Rastawicki, W.; Jagielski, M.; Kałużewski, S.; Gierczyñski, R. // European Journal of Clinical Microbiology & Infectious Diseases;May2002, Vol. 21 Issue 5, p417 

    This article focuses on a study which evaluated a latex agglutination test prepared for the detection of IgM and IgG antibodies to Mycoplasma pneumoniae by comparing it with the ELISA-IgM/IgG test and the conventional CF test. Among the 66 serum samples tested, 36 were positive by the complement...

  • Detection of Leptospires serogroups, Which Are Common Causes of Human Acute Leptospirosis in Guilan, Northern Iran. Honarmand, H. R.; Eshraghi, S. S. // Iranian Journal of Public Health;2011, Vol. 40 Issue 1, p107 

    Background: This study is performed to reveal most common species and subspecies of leptospires that are main causes of human leptospirosis in Guilan, Northern Province of Iran. Methods: We performed IgM-ELISA and MAT on 282 blood samples from patients who attended to 3 hospitals in the flat...

  • Epidemiology of paediatric visceral leishmaniasis in Hebron district, Palestine Amro, Ahmad; Azmi, Kifaya; Schönian, Gabriele; Nasereddin, Abedelmajeed; Alsharabati, Mohamed Barakat; Sawalha, Samer; Hamarsheh, Omar; Ereqat, Suhair; Abdeen, Ziad // Transactions of the Royal Society of Tropical Medicine & Hygiene;Jul2009, Vol. 103 Issue 7, p731 

    Summary: Seventy-six cases of visceral leishmaniasis (VL) were reported from the Hebron district of the West Bank, Palestine between 1993 and 2007. All cases were in children less than 9 years old (median age 2 years). The average number of cases was 5.06/year and the average annual incidence...

Share

Read the Article

Courtesy of

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics