Short Report: Tissue-specific Expression Profiles of the Uncoupling Protein Family in Normal Control Mice and Genetically ob/ob Mice

Chun-Mei Zhang; Min Zhang; Jin-Gai Zhu; Chen-Bo Ji; Chun Zhu; Chun-Zhao Kou; Da-Ni Qin; Mei-Ling Tong; Xi-Rong Guo
June 2010
Journal of Bioenergetics & Biomembranes;Jun2010, Vol. 42 Issue 3, p255
Academic Journal
Uncoupling proteins (UCPs) located in the inner mitochondrial membrane are involved in the regulation of energy balance. Thus far, 5 UCP isoforms have been identified, but controversies exist in the research focused on the function of the UCPs (except UCP1) in the pathogenesis of obesity. Because of the known cross-reactivity of the antibodies presently available for the detection of UCP proteins, this study systematically analyzed the differential tissue expression profiles of the 5 UCP isoforms in lean control mice and ob/ob mice by using real-time polymerase chain reaction (PCR) analysis. The results show that the tissue-specific expression patterns of individual isoforms in normal and ob/ob mice are considerably different; this will provide new insights into the functions of UCPs in the pathogenesis of genetic obesity.


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