TITLE

EST sequencing and gene expression profiling of cultivated peanut (Arachis hypogaea L.)

AUTHOR(S)
Bi, Yu-Ping; Liu, Wei; Xia, Han; Su, Lei; Zhao, Chuan-Zhi; Wan, Shu-Bo; Wang, Xing-Jun
PUB. DATE
October 2010
SOURCE
Genome;Oct2010, Vol. 53 Issue 10, p832
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Peanut (Arachis hypogaea L.) is one of the most important oil crops in the world. However, biotechnological based improvement of peanut is far behind many other crops. It is critical and urgent to establish the biotechnological platform for peanut germplasm innovation. In this study, a peanut seed cDNA library was constructed to establish the biotechnological platform for peanut germplasm innovation. About 17 000 expressed sequence tags (ESTs) were sequenced and used for further investigation. Among which, 12.5% were annotated as metabolic related and 4.6% encoded transcription or post-transcription factors. ESTs encoding storage protein and enzymes related to protein degradation accounted for 28.8% and formed the largest group of the annotated ESTs. ESTs that encoded stress responsive proteins and pathogen-related proteins accounted for 5.6%. ESTs that encoded unknown proteins or showed no hit in the GenBank nr database accounted for 20.1% and 13.9%, respectively. A total number of 5066 EST sequences were selected to make a cDNA microarray. Expression analysis revealed that these sequences showed diverse expression patterns in peanut seeds, leaves, stems, roots, flowers, and gynophores. We also analyzed the gene expression pattern during seed development. Genes that were upregulated (>=twofold) at 15, 25, 35, and 45 days after pegging (DAP) were found and compared with 70 DAP. The potential value of these genes and their promoters in the peanut gene engineering study is discussed. L'arachide (Arachis hypogaea L.) est l'une des oléagineuses les plus importantes dans le monde. Malgré cela, la contribution des biotechnologies à l'amélioration génétique chez cette espèce a été beaucoup moindre que chez d'autres espèces. Il est ainsi critique et urgent d'établir une plateforme biotechnologique permettant l'innovation au sein des ressources génétiques de l'arachide. Dans ce travail, une banque d'ADNc de graines de l'arachide a été produite pour fonder l'assise d'une plateforme biotechnologique d'innovation chez l'arachide. Environ 17 000 EST ont été séquencés et employés lors d'analyses subséquentes. Parmi ceux-ci, 12,5% ont été annotés comme étant liés au métabolisme et 4,6% des EST codaient pour des facteurs transcriptionnels ou post-transcriptionnels. Les EST codant pour des protéines de réserve ou pour des enzymes impliquées dans la dégradation des protéines formaient le plus grand groupe et représentaient 28,8% des EST annotés. Les EST codant pour des protéines de réponse aux stress ou aux pathogènes constituaient 5,6% du total. Les EST codant pour des protéines inconnues et ceux codant pour des protéines sans homologues au sein de GenBank constituaient respectivement 20,1% et 13,9% du total. Une collection de 5066 séquences d'EST a été retenue pour fabriquer une puce d'ADNc. Une analyse transcriptomique a révélé que ces séquences montraient divers motifs d'expression chez les graines, les feuilles, les tiges, les racines, les fleurs et les gynophores de l'arachide. Les auteurs ont également analysé l'expression génique au cours du développement des graines. Des gènes qui étaient sur-exprimés (>2 fois) à 15, 25, 35 et 45 jours après fécondation par rapport au niveau enregistré à 70 jours ont été identifiés. Les auteurs discutent de l'utilité potentielle de ces gènes et de leurs promoteurs pour des fins d'ingénierie génétique chez l'arachide.
ACCESSION #
54550290

 

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