Establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation

Parameswari, Chidambaram; Sripriya, Rajasekaran; Veluthambi, Karuppannan
October 2010
In Vitro Cellular & Developmental Biology Plant;Oct2010, Vol. 46 Issue 5, p395
Academic Journal
In an analysis of 339 independent T transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the T generation. The albino phenotype segregated as a monogenic recessive trait. Southern blot analysis of the green and albino plants in the T generation confirmed that the albino trait and the T-DNA insertion events were unlinked. Segregation of the albino trait from the transgenic trait in the lines O54 and O36 was confirmed in T and T generations, respectively. Homozygous transgenic plants free from the albino trait were also identified. In the double-copy transgenic line C18, we genetically separated the two transgenic loci, out-segregated the albino locus from both transgene loci, and identified homozygous plants for each of the transgenic events by Southern blot analysis in the T generation itself. Thus, we demonstrate that when an albino trait appears in the T generation and is unlinked to a transgene locus, the albino locus can be segregated from the transgene locus and homozygous transgenic lines free from albinos can be established.


Related Articles

  • The promoter of a rice glycine-rich protein gene, Osgrp-2, confers vascular-specific expression in transgenic plants. Zong-Zhi Liu; Jian-Long Wang; Xun Huang; Wei-Hui Xu; Zhao-Ming Liu; Rong-Xiang Fang // Planta;Mar2003, Vol. 216 Issue 5, p824 

    The genomic sequence of a rice (Oryza sativa L.) glycine-rich protein (GRP) gene, designated Osgrp-2, has been previously determined (GenBank U40708). Primer extension analysis indicated that transcription starts 47 bp upstream of the translation start codon. To gain an insight into the...

  • A one-time inducible transposon for creating knockout mutants. Kuan-Te Li; Ya-Lin Lin; Ji-Ying Huang; Wen-Ya Li; Yuh-Chyang Charng // Molecular Breeding;Aug2008, Vol. 22 Issue 1, p85 

    The maize transposon Ac can move to a new location within the genome to create knockout mutants in transgenic plants. In rice, Ac transposon is very active but sometimes undergoes further transposition and leaves an empty mutated gene. Therefore, we developed a one-time transposon system by...

  • Expression of a plant defensin in rice confers resistance to fungal phytopathogens. Jha, Sanjay; Chattoo, Bharat B. // Transgenic Research;Jun2010, Vol. 19 Issue 3, p373 

    Transgenic rice ( Oryza sativa L. cv. Pusa basmati 1), overexpressing the Rs-AFP2 defensin gene from the Raphanus sativus was generated by Agrobacterium tumefaciens-mediated transformation. Expression levels of Rs-AFP2 ranged from 0.45 to 0.53% of total soluble protein in transgenic plants. It...

  • Over-expression of Arabidopsis FT gene reduces juvenile phase and induces early flowering in ornamental gentian plants. Nakatsuka, Takashi; Abe, Yoshiko; Kakizaki, Yuko; Kubota, Akiko; Shimada, Norimoto; Nishihara, Masahiro // Euphytica;Jul2009, Vol. 168 Issue 1, p113 

    Ornamental gentian plants are perennial and have a juvenile period of over 1 year before flowering. We transformed gentian plants with a construct comprising the Arabidopsis FLOWERING LOCUS T ( FT) gene (encoding a major component protein of the flowering hormone �florigen�) under the...

  • Mutations in the rice liguleless gene result in a complete loss of the auricle, ligule, and laminar joint. Jinwon Lee; Jong-Jin Park; Song Kim; Jieun Yim; Gynheung An // Plant Molecular Biology;Nov2007, Vol. 65 Issue 4, p487 

    Abstract  The area between the upper part of the leaf sheath and the basal portion of the leaf blade contains several specialized organs, such as the laminar joint, auricle and ligule. Here we report the identification of T-DNA insertional mutant lines that lack all of these organs. The...

  • Healthier Frankenfoods? Regalado, Antonio // Technology Review;Mar/Apr2000, Vol. 103 Issue 2, p32 

    Discusses issues involved in the genetic engineering of agricultural crops. Advantages offered by a genetically engineered strain of rice that produces beta-carotene; Opposition to genetically engineered foods; Lack of a clear benefit to consumers in first generation genetically engineered food...

  • Excision of a selective marker in transgenic rice using a novel Cre/ loxP system controlled by a floral specific promoter. Chengcai Chu // Transgenic Research;Dec2008, Vol. 17 Issue 6, p1035 

    Abstract  Based on the Cre/loxP system, we have developed a novel marker-free system mediating a direct auto-excision of loxP-flanked marker genes from T1 transgenic rice without any treatment or further offspring crossing. To achieve this, the floral-specific promoter OsMADS45...

  • Cre/lox site-specific recombination controls the excision of a transgene from the rice genome. Hoa, T. T. C.; Bong, B. B.; Huq, E.; Hodges, T. K. // Theoretical & Applied Genetics;Mar2002, Vol. 104 Issue 4, p518 

    The Cre/lox site-specific recombination controls the excision of a target DNA segment by recombination between two lox sites flanking it, mediated by the Cre recombinase. We have studied the functional expression of the Cre/lox system to excise a transgene from the rice genome. We developed...

  • The relationship between homozygous and hemizygous transgene expression levels over generations in populations of transgenic rice plants. James, V. A.; Avart, C.; Worland, B.; Snape, J. W.; Vain, P. // Theoretical & Applied Genetics;Mar2002, Vol. 104 Issue 4, p553 

    Segregating T1, T2 and T3 transgenic rice populations, derived from independent particle-bombardment-mediated transformation events were examined in order to assess the effect of gene dosage on transgene expression levels and stability. The expression level of the unselected β-glucuronidase...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics