Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells. First Successful Latin-American Report

Mardones, Rodrigo; Martínez, Rafael; Paredes, María Jose; Zalaquett, Eugenio
September 2010
International Journal of Morphology;2010, Vol. 28 Issue 3, p749
Academic Journal
Osteoarthritis is the more frequent cause of disability in adult people and it is associated to cartilage degeneration of affected joints. This cartilage has a limited ability to repair. Several treatments have been tested including the use of Mesenchymal Stem Cells. These cells are an attractive source for cartilage repair because of their availability to chodrogenic differentiation by progressing sequentially through the expression of cartilage specific extracelullar matrix molecules, as in the embryologic human development. The aim is to obtain, culture and differentiate rabbit Bone Marrow derived Mesenchymal Stem Cells in vitro to chondral lineage. By differential centrifugation the mononuclear cell level was obtained from rabbit bone marrow samples. This level was cultured until 70% confluence. Chondrogenic differentiation was performed in an aggregate culture system with TGF-b1. Sample quantity, culture efficiency, confluence time of cultures and differentiation quality were all evaluated. An average sample of 14.5 ml per side was obtained, culture efficiency was 80%, and average confluence time (70%) was 18 days. Differentiation culture had an 80% efficiency and optimal differentiation quality. Rabbit Bone Marrow derived Mesenchymal Stem Cells culture is a reproducible technique and by the use of an adequate methodology chondrogenic cells can be obtained in vitro. This model permits the study of chondral differentiation process and could have direct clinical application. This is the first successful Latin-American report in Mesenchymal Stem Cells culture and chondrogenic differentiation.


Related Articles

  • In vitro differentiation of mesenchymal stem cells into endothelial cells. Siddiqui, Irfan Ahmed // Der Pharmacia Sinica;2013, Vol. 4 Issue 1, p106 

    The aim of the investigation was to detect the in vitro differentiation of mesenchymal stem cells (MSCs) into cells of the endothelial lineage. MSCs were generated from mononuclear bone marrow cells from healthy donors separated by density gradient centrifugation. Cells were characterized by...

  • In vitro gene and chromosome characterization of expanded bone marrow mesenchymal stem cells for musculo-skeletal applications. ROSETI, L.; SERRA, M.; CANELLA, E.; MUNNO, C.; TOSI, A.; ZUNTINI, M.; PANDOLFI, M.; SANGIORGI, L.; BISO, R.; PITTALIS, M. C.; BINI, C.; PELOTTI, S.; GASBARRINI, A.; BORIANI, L.; BASSI, A.; GRIGOLO, B. // European Review for Medical & Pharmacological Sciences;Dec2014, Vol. 18 Issue 23, p3702 

    OBJECTIVE: A number of studies have shown the role of expanded Bone Marrow-derived Mesenchymal Stem Cells in the repair and regeneration of musculo-skeletal tissues. The current European regulations define in vitro expanded cells for clinical purposes as substantially manipulated and include...

  • Myogenic Potential of Whole Bone Marrow Mesenchymal Stem Cells In Vitro and In Vivo for Usage in Urinary Incontinence. Gunetti, Monica; Tomasi, Simone; Giammò, Alessandro; Boido, Marina; Rustichelli, Deborah; Mareschi, Katia; Errichiello, Edoardo; Parola, Maurizio; Ferrero, Ivana; Fagioli, Franca; Vercelli, Alessandro; Carone, Roberto; Câmara, Niels Olsen Saraiva // PLoS ONE;Sep2012, Vol. 7 Issue 9, Special section p1 

    Urinary incontinence, defined as the complaint of any involuntary loss of urine, is a pathological condition, which affects 30% females and 15% males over 60, often following a progressive decrease of rhabdosphincter cells due to increasing age or secondary to damage to the pelvic floor...

  • Mesenchymal stem cells immunosuppressive properties: is it specific to bone marrow-derived cells? Jorgensen, Christian // Stem Cell Research & Therapy;2010, Vol. 1 Issue 2, p1 

    Based on their capacity to suppress immune responses, multipotent mesenchymal stromal cells (MSCs) are intensively studied for regenerative medicine. Moreover, MSCs have paracrine effects, including immunomodulation that occurs through the secretion of soluble mediators, including nitric oxide...

  • Protection of mesenchymal stem cells on acute kidney injury. JING-JIE ZHAO; JUN-LI LIU; LING LIU; HONG-YING JIA // Molecular Medicine Reports;2014, Vol. 9 Issue 1, p91 

    The aim of the present study was to determine the protection of allogeneic bone marrow mesenchymal stem cells (BMSCs) on an ischaemia/reperfusion (I/R)-induced acute kidney injury (AKI) rat model and to investigate the underlying mechanisms. The BMSCs were isolated and cultured from adult...

  • All-arthroscopic AMIC procedure for repair of cartilage defects of the knee. Piontek, Tomasz; Ciemniewska-Gorzela, Kinga; Szulc, Andrzej; Naczk, Jakub; Słomczykowski, Michał // Knee Surgery, Sports Traumatology, Arthroscopy;May2012, Vol. 20 Issue 5, p922 

    Purpose: Bone marrow mesenchymal stem cells were introduced into clinical practice due to their ability to differentiate into many types of cells. Autologous matrix-induced chondrogenesis (AMIC) combines the microfracture method with matrix-based techniques that utilizes a collagen membrane to...

  • Calcite Biohybrids as Microenvironment for Stem Cells. Astachov, Liliana; Nevo, Zvi; Vago, Razi // Polymers (20734360);Jun2012, Vol. 4 Issue 2, p1065 

    A new type of composite 3D biomaterial that provides extracellular cues that govern the differentiation processes of mesenchymal stem cells (MSCs) has been developed. In the present study, we evaluated the chondrogenecity of a biohybrid composed of a calcium carbonate scaffold in its calcite...

  • MicroRNA-92a-3p Regulates Aggrecanase-1 and Aggrecanase-2 Expression in Chondrogenesis and IL-1β-Induced Catabolism in Human Articular Chondrocytes. Mao, Guping; Wu, Peihui; Zhang, Ziji; Zhang, Zhiqi; Liao, Weiming; Li, Yukang; Kang, Yan // Cellular Physiology & Biochemistry (Karger AG);Dec2017, Vol. 44 Issue 1, p38 

    Background/Aims: Aggrecanase-1 (ADAMTS-4) and aggrecanase-2 (ADAMTS-5) are secreted enzymes belonging to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family that play significant roles in the progression of osteoarthritis (OA). Here, we aimed to determine whether...

  • CORRECTION: Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm. Dongyan SHI; Dan MA; Feiqing DONG; Chen ZONG; Liyue LIU; Dan SHEN; Wenji YUAN; Xiangmin TONG; Hengwu CHEN; Jinfu WANG // Bioscience Reports;Dec2010, Vol. 30 Issue 6, p455 

    A correction to the article "Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm," by Dongyan Shi and colleagues in the 30th volume of the "Bioscience Reports" journal is presented.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics