TITLE

Fibroblast Growth Factor 10 Induces Proliferation and Differentiation of Human Primary Cultured Keratinocytes

AUTHOR(S)
Marchese, Cinzia; Felici, Alessandra; Visco, Vincenzo; Lucania, Giuseppe; Igarashi, Makoto; Picardo, Mauro; Frati, Luigi; Torrisi, Maria Rosaria
PUB. DATE
April 2001
SOURCE
Journal of Investigative Dermatology;Apr2001, Vol. 116 Issue 4, p623
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Fibroblast growth factor 10 is a novel member of the fibroblast growth factor family, which is involved in morphogenesis and epithelial proliferation. It is highly homologous to the keratinocyte growth factor (or fibroblast growth factor 7), a key mediator of keratinocyte growth and differentiation. Both fibroblast growth factor 10 and keratinocyte growth factor bind with high affinity to the tyrosine kinase keratinocyte growth factor receptor. Here we analyzed the effect of fibroblast growth factor 10 on primary cultures of human keratinocytes, grown in chemically defined medium, and we compared the proliferative and differentiative cell responses to fibroblast growth factor 10 with those induced by keratinocyte growth factor and epidermal growth factor. Cell counting, 5-bromo-2′-deoxyuridine incorporation, and western blot analysis showed that fibroblast growth factor 10, similarly to keratinocyte growth factor, not only is a potent mitogen for human keratinocytes, but also promotes the expression of both early differentiation markers K1 and K10 and late differentiation marker filaggrin in response to the Ca2+ signal, and seems to sustain the proliferative activity in suprabasal stratified cells. Immunoprecipitation/western blot analysis revealed that fibroblast growth factor 10, similarly to keratinocyte growth factor, is able to induce tyrosine phosphorylation of keratinocyte growth factor receptor and of cellular substrates such as PLCγ.
ACCESSION #
5724178

 

Related Articles

  • Keratinocyte growth factor activates p38 MAPK to induce stress fibre formation in human prostate DU145 cells. Mehta, Piyush B; Robson, Craig N; Neal, David E; Leung, Hing Y // Oncogene;8/30/2001, Vol. 20 Issue 38, p5359 

    FGF7/Keratinocyte growth factor (KGF) regulates the differentiation and development of the prostate epithelium, while over-expression of FGF8 and FGF1 are implicated in carcinogenesis of the prostate. We tested the hypothesis that different members of the FGF family function through different...

  • MiR-125b, a MicroRNA Downregulated in Psoriasis, Modulates Keratinocyte Proliferation by Targeting FGFR2. Ning Xu; Brodin, Petter; Tianling Wei; Meisgen, Florian; Eidsmo, Liv; Nagy, Nikoletta; Kemeny, Lajos; Ståhle, Mona; Sonkoly, Enikö; Pivarcsi, Andor // Journal of Investigative Dermatology;Jul2011, Vol. 131 Issue 7, p1521 

    MicroRNAs (miRNAs) are short, single-stranded, noncoding RNAs that play important roles in the regulation of gene expression. We previously identified a characteristic miRNA expression profile in psoriasis, distinct from that of healthy skin. One of the most downregulated miRNAs in psoriasis...

  • First-Class Delivery: Getting Growth Factors to Their Destination. Davidson, Jeffrey M. // Journal of Investigative Dermatology;Jun2008, Vol. 128 Issue 6, p1360 

    Growth factor bioavailability in therapeutic applications such as wound healing is limited by extracellular matrix sequestration, proteolysis, and clearance. Local, transient delivery by gene transfer is an attractive concept. Many transfection strategies are available, and adenoviral vectors...

  • Induction of Keratinocyte Growth Factor Expression Is reduced and Delayed During Wound Healing in the Genetically Diabetic Mouse. Werner, Sabine; Breeden, Matthew; Hübner, Griseldis; Greenhalgh, David G.; Longaker, Michael T. // Journal of Investigative Dermatology;Oct94, Vol. 103 Issue 4, p469 

    We have recently demonstrated induction of expression of several members of the fibroblast growth factor family during wound healing, particularly for keratinocyte growth factor, which was more than 150-fold induced within 24 h after injury. To assess whether wound-healing disorders are...

  • ORIGINAL ARTICLE Crucial Effects of Fibroblasts and Keratinocyte Growth Factor on Morphogenesis of Reconstituted Human Oral Epithelium. Costea, Daniela Elena; Loro, Lado Lako; Dimba, Elisabeth Anne Okumo; Karsten, Vintermyr, Olav; Johannessen, Anne Christine // Journal of Investigative Dermatology;Dec2003, Vol. 121 Issue 6, p1479 

    The connective tissue is known to have a general supportive effect for the development of the overlying epithelium; however, the more specific effects of fibroblasts and the involvement of their product, keratinocyte growth factor, on oral epithelial morphogenesis have not yet been addressed....

  • Increased expression of fibroblast growth factors in segmental renal dysplasia. Shima, H.; Tazawa, H.; Puri, P. // Pediatric Surgery International;May2000, Vol. 16 Issue 4, p306 

    Renal dysplasia (RD) is a disorganized development of renal parenchyma that results in a deficit of functional renal tissue. Dysplastic renal tissue is characterized by primitive tubular epithelium associated with increased mesenchyme. Several polypeptide growth factors (GF), which interact with...

  • A Modeling Approach to Study the Effect of Cell Polarization on Keratinocyte Migration. Fuhr, Matthias Jörg; Meyer, Michael; Fehr, Eric; Ponzio, Gilles; Werner, Sabine; Herrmann, Hans Jürgen // PLoS ONE;Feb2015, Vol. 10 Issue 2, p1 

    The skin forms an efficient barrier against the environment, and rapid cutaneous wound healing after injury is therefore essential. Healing of the uppermost layer of the skin, the epidermis, involves collective migration of keratinocytes, which requires coordinated polarization of the cells. To...

  • Exogenous Addition of a C-Xylopyranoside Derivative Stimulates Keratinocyte Dermatan Sulfate Synthesis and Promotes Migratio. Muto, Jun; Naidu, Nandita Natasha; Yamasaki, Kenshi; Pineau, Nathalie; Breton, Lionel; Gallo, Richard L. // PLoS ONE;2011, Vol. 6 Issue 10, p1 

    As C-Xyloside has been suggested to be an initiator of glycosaminoglycan (GAG) synthesis, and GAGs such as Dermatan sulfate (DS) are potent enhancers of fibroblast growth factor (FGF) - 10 action, we investigated if a C-Xylopyranoside derivative, (C-β-D-xylopyranoside-2-hydroxy-propane,...

  • Hypoxia regulates CD9-mediated keratinocyte migration via the P38/ MAPK pathway. Xupin Jiang; Xiaowei Guo; Xue Xu; Miao Teng; Chong Huang; Dongxia Zhang; Qiong Zhang; Jiaping Zhang; Yuesheng Huang // Scientific Reports;9/12/2014, p1 

    Keratinocyte migration is an early event in the wound healing process. Although we previously found that CD9 downregulation is required for the keratinocyte migration during wound repair, the mechanism of how CD9 expression is regulated remains unclear. Here, we observed the effect of hypoxia...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics