Expression and characterization of two new alkane-inducible cytochrome P450s from Trichoderma harzianum

del Carratore, R.; Gervasi, P. G.; Contini, M. P.; Beffy, P.; Maserti, B. E.; Giovannetti, G.; Brondolo, A.; Longo, V.
June 2011
Biotechnology Letters;Jun2011, Vol. 33 Issue 6, p1201
Academic Journal
n-Dodecane and fatty acids were good inducers of cytochrome P450 (CYP) and the ω-hydroxylase of lauric acid, which is a marker for ω-hydroxylation of n-alkanes, in Trichoderma harzianum. A cDNA, containing an ORF of 1520 bp, encoding a CYP52 of 520 amino acids, was isolated by RACE. Another n-alkane-inducible CYP was identified by LLC-MS/MS analysis of a microsomal protein band induced by n-dodecane in a library of T. harzianum. This suggests that T. harzianum has a CYP-dependent conversion of alkanes to fatty acids allowing their incorporation into lipids.


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