Comparison of stress-induced PRINS gene expression in normal human keratinocytes and HaCaT cells

Bari, Lilla; Bacsa, Sarolta; Sonkoly, Enikő; Bata-Csörgő, Zsuzsanna; Kemény, Lajos; Dobozy, Attila; Széll, Márta
December 2011
Archives of Dermatological Research;Dec2011, Vol. 303 Issue 10, p745
Academic Journal
Psoriasis is a chronic inflammatory skin disease that affects approximately 2-4% of the population. We recently described a novel non-coding RNA, psoriasis susceptibility related RNA gene induced by stress (PRINS), that was overexpressed in non-lesional psoriatic epidermis, and its expression was induced by various stress factors such as serum starvation, contact inhibition, ultraviolet (UV)-B irradiation, viral infection and translational inhibition in HaCaT cells. In the present work we set out to compare the stress and microbial agent-induced PRINS expression in normal human keratinocytes (NHKs) and HaCaT cells. Since nuclear factor-κB (NF-κB) is involved in the cellular stress response, we sought to explore whether there is a connection between the NF-κB and PRINS-mediated signal transduction pathways in NHKs and HaCaT cells. We found that the PRINS expression responded differentially to various stress signals and microbial agents in HaCaT cells and in NHKs: after translational inhibition and UV-B treatment, similar induction of PRINS expression occurred with different time courses while after microbial agent treatment, the PRINS expression was significantly induced in HaCaT cells, whereas we could not detect similar changes in NHKs. To explore whether the known NF-κB abnormalities in HaCaT cells could be related to this differential PRINS expression, we silenced the PRINS gene expression with small interfering RNA (siRNA) in both HaCaT cells and in NHKs and monitored NF-κB signal transduction after lipopolysaccharide (LPS) treatment. Silencing of PRINS had no effect on LPS-induced NF-κB activity either in HaCaT cells or in NHKs. Our results indicate that PRINS probably affects keratinocytes functions independently of NF-κB signalling.


Related Articles

  • Innate immunity in the pathogenesis of psoriasis. Sweeney, Cheryl; Tobin, Ann-Marie; Kirby, Brian // Archives of Dermatological Research;Dec2011, Vol. 303 Issue 10, p691 

    Psoriasis is a common, immune-mediated inflammatory skin disorder. T helper(h)1 and Th17 lymphocytes contribute to the pathogenesis of psoriasis through the release of inflammatory cytokines that promote further recruitment of immune cells, keratinocyte proliferation and sustained inflammation....

  • Glucocorticoid Receptor Localizes to Adherens Junctions at the Plasma Membrane of Keratinocytes. Stojadinovic, Olivera; Sawaya, Andrew; Pastar, Irena; Tomic-Canic, Marjana // PLoS ONE;Apr2013, Vol. 8 Issue 4, p1 

    Glucocorticoids are important regulators of epidermal tissue homeostasis. As such, their clinical applications are widespread, ranging from inflammatory skin disorders to keloids and cancer. Glucocorticoids exert their effect by binding to glucocorticoid receptor (GR) which translocates to the...

  • Syk Mediates IL-17-Induced CCL20 Expression by Targeting Act1-Dependent K63-Linked Ubiquitination of TRAF6. Wu, Nan-Lin; Huang, Duen-Yi; Tsou, Hsin-Ni; Lin, Ying-Cing; Lin, Wan-Wan // Journal of Investigative Dermatology;Feb2015, Vol. 135 Issue 2, p490 

    IL-17 has an important role in the immunopathogenesis of autoimmune diseases, and spleen tyrosine kinase (Syk) has been implicated as a critical molecule in the signaling pathways of various immunoreceptors. Chemokine (C-C motif) ligand 20 (CCL20) interacts with chemokine (C-C motif) receptor 6...

  • IRAK1-Dependent Signaling Mediates Mortality in Polymicrobial Sepsis. Chandra, Rachna; Federici, Stephanie; Bishwas, Tripti; Németh, Zoltán; Deitch, Edwin; Thomas, James; Spolarics, Zoltán // Inflammation;Dec2013, Vol. 36 Issue 6, p1503 

    Interleukin-1 receptor-associated kinase (IRAK1) is a key regulatory protein in TLR/IL1R-mediated cell activation during inflammatory response. Studies indicated that pending on the nature of the used inflammatory model, downregulation of IRAK1 may be beneficial or detrimental. However, the role...

  • Amygdalin Analogues Inhibit IFN-γ Signalling and Reduce the Inflammatory Response in Human Epidermal Keratinocytes. Paoletti, Iole; Gregorio, Vincenza; Baroni, Adone; Tufano, Maria; Donnarumma, Giovanna; Perez, Juan // Inflammation;Dec2013, Vol. 36 Issue 6, p1316 

    Peptide T (PT), an octapeptide fragment located in the V2 region of the HIV-1 gp120-coating protein, appears to be beneficial in the treatment of psoriasis. Our previous investigations suggest that keratinocytes play a key role in conditioning the therapeutic effects of PT in psoriasis. The aim...

  • Spontaneous Atopic Dermatitis-Like Symptoms in a/a ma ft/ma ft/J Flaky Tail Mice Appear Early after Birth. Kypriotou, Magdalini; Boéchat, Cloé; Huber, Marcel; Hohl, Daniel // PLoS ONE;Jul2013, Vol. 8 Issue 7, p1 

    Loss-of-function mutations in human profilaggrin gene have been identified as the cause of ichthyosis vulgaris (IV), and as a major predisposition factor for atopic dermatitis (AD). Similarly, flaky tail (a/a ma ft/ma ft/J) mice were described as a model for IV, and shown to be predisposed to...

  • Melanocortin-3-receptor promoter polymorphism associated with tuberculosis susceptibility does not influence protein expression. Eggert, Marlene; Pfob, Martina; Steinlein, Ortrud K. // BMC Research Notes;2013, Vol. 6 Issue 1, p1 

    Background: The melanocortin-3-receptor (MC3R) is a member of the G-protein coupled receptor family that mediate cellular response through the cyclic adenosine monophosphate signalling pathway. In the promoter region of MC3R the polymorphism rs6127698 has previously been shown to be strongly...

  • Increased Expression of the Dyslexia Candidate Gene DCDC2 Affects Length and Signaling of Primary Cilia in Neurons. Massinen, Satu; Hokkanen, Marie-Estelle; Matsson, Hans; Tammimies, Kristiina; Tapia-Páez, Isabel; Dahlström-Heuser, Vanina; Kuja-Panula, Juha; Burghoorn, Jan; Jeppsson, Kristian E.; Swoboda, Peter; Peyrard-Janvid, Myriam; Toftga°rd, Rune; Castrén, Eero; Kere, Juha // PLoS ONE;2011, Vol. 6 Issue 6, p1 

    DCDC2 is one of the candidate susceptibility genes for dyslexia. It belongs to the superfamily of doublecortin domain containing proteins that bind to microtubules, and it has been shown to be involved in neuronal migration. We show that the Dcdc2 protein localizes to the primary cilium in...

  • Comparative transcriptome analysis of yeast strains carrying slt2, rlm1, and pop2 deletions. Becerra, M.; Lombard�a, L. J.; Lamas-Maceiras, M.; Canto, E.; Rodr�guez-Belmonte, E.; Cerd�n, M. E. // Genome;Feb2011, Vol. 54 Issue 2, p99 

    The function of the genes SLT2 (encoding the Mpk1 protein), RLM1, and POP2 have previously been related to several stress responses in yeasts. DNA arrays have been used to identify differences among the transcriptomes of a Saccharomyces cerevisiae wild type strain and its derivative ?slt2,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics