Expression of nestin and nerve growth factors in adipose-derived mesenchymal stem cells

Ghorbanian, M. T.; Haji-Ghasem-Kashani, M.; Hossein-Pour, L.; Mirzaiyan, L.
January 2012
Feyz Journal of Kashan University of Medical Sciences;Jan2012, Vol. 16 Issue 1, p322
Academic Journal
Background: Adipose tissue represents an accessible source of mesenchymal stem cells (Adipose tissue derived mesenchymal stem cells-ADSCs). ADSCs could be differentiated into various mesenchymal stem cells (e.g. chondrocytes, adipocytes, osteoblasts, and neuronal lineages). Neurotrophic factors are diffusible polypeptides that have a critical role in survival, proliferation and differentiation of stem cells. This study aimed to evaluate the proliferative capacity and the expression of some neurotrophic factors such as brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), glial cell-derived neurotrophic factor (GDNF), neurotrophin-3 (NT-3), neurotrophin-4 (NT-4), nerve growth factor (NGF) and the expression of nestin in ADSCs. Materials and Methods: Rat ADSCs were isolated from the subcutaneous adipose tissue using mechanical and enzymatic digestion. These cells were cultured in aMEM supplemented with 10% fetal bovine serum. ADSCs of the third passage were evaluated by immunocytochemistry to detect the cell surface markers of CD90 and nestin. Reverse transcription-polymerase chain reaction (RT-PCR) was used to study the expression of the above-mentioned neurotrophic factors in the ADSCs. Moreover, adipogenic and chondrogenic differentiation of ADSCs were induced in-vitro. Results: The assessment of ADSCs identity and purity showed that 90% and 80% of the stem cells were positive for CD90 marker and nestin, respectively. According to RT-PCR results, the above-mentioned neurotrophic factors were expressed in these stem cells. Furthermore, a small number of cells were positive for cresyl violet staining. Conclusion: Adipose tissue contains a stem cell population that seems to be a good multipotential cell candidate for cell replacement therapy.


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