TITLE

İndüklenmiş Pluripotent Kök Hücreler ve Uygulamaları

AUTHOR(S)
Sevim, Handan; Gürpinar, Özer Aylin
PUB. DATE
January 2012
SOURCE
Marmara Medical Journal;2012, Vol. 25 Issue 1, p5
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Pluripotency is a property of a cell that allows it to develop as any of the cell types of the body. Embryonic stem cells (ESCs) are unique cells in the organism and they have a pluripotent capacity. Induced pluripotent stem cells (IPSCs) is a term that describes somatic cells having a pluripotent capacity induced by the viral transfection of special genes. This term was firstly used in 2006 by Takahashi and Yamanaka in their experimental work. c-Myc, Sox-2, Oct ¾ and Klf-4 genes are used for the transfection of somatic cells in order to obtain IPSCs. IPSC colonies are produced by using a successful transfection process. IPSCs have pluripotent stem cell specialities like growing potential in a culture system, having a DNA methylation pattern, an ability to form teratomas, to generate three germ line components and to generate chimeric organisms which pluripotent ESCs have. Concerning the ethical problems of working with the ESCs, IPSCs can be a unique source for pluripotency studies. IPSCs with their pluripotent capacity can be used for cell therapies in diseases which have irreversible cell defects. IPSCs can also be used to form autologus implants with no immune response. Therefore, IPSCs can be used for cell therapies, drug research or disease models. In this review, we give some information about obtaining IPSCs and today's research areas that have been opened by the use of these cells.
ACCESSION #
70146656

 

Related Articles

  • Embryonic stem cell and induced pluripotent stem cell: an epigenetic perspective. Liang, Gaoyang; Zhang, Yi // Cell Research;Jan2013, Vol. 23 Issue 1, p49 

    Pluripotent stem cells, like embryonic stem cells (ESCs), have specialized epigenetic landscapes, which are important for pluripotency maintenance. Transcription factor-mediated generation of induced pluripotent stem cells (iPSCs) requires global change of somatic cell epigenetic status into an...

  • Zfp296 Is a Novel, Pluripotent-Specific Reprogramming Factor. Fischedick, Gerrit; Klein, Diana C.; Wu, Guangming; Esch, Daniel; Höing, Susanne; Han, Dong Wook; Reinhardt, Peter; Hergarten, Kerstin; Tapia, Natalia; Schöler, Hans R.; Sterneckert, Jared L. // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    Expression of the four transcription factors Oct4, Sox2, Klf4, and c-Myc (OSKM) is sufficient to reprogram somatic cells into induced pluripotent stem (iPSCs). However, this process is slow and inefficient compared with the fusion of somatic cells with embryonic stem cells (ESCs), indicating...

  • Clinical applications of patient-specific induced pluripotent stem cells in cardiovascular medicine. Yingzi Oh; Heming Wei; Dongrui Ma; Xiaoming Sun; Liew, Reginald // Heart;Mar2012, Vol. 98 Issue 6, p443 

    The emergence of induced pluripotent stem cell (iPSC) technology has had a great impact on the field of medicine ever since the ground-breaking discovery in 2006 that overexpression of four specific transcription factors was able to turn back the developmental clock of somatic cells into an...

  • Role of Oct4 in maintaining and regaining stem cell pluripotency. Guilai Shi; Ying Jin // Stem Cell Research & Therapy;2010, Vol. 1 Issue 5, p1 

    Pluripotency, a characteristic of cells in the inner cell mass of the mammalian preimplantation blastocyst as well as of embryonic stem cells, is defined as the ability of a cell to generate all of the cell types of an organism. A group of transcription factors is essential for the establishment...

  • Experimental approaches for the generation of induced pluripotent stem cells. Sommer, Cesar A.; Mostoslavsky, Gustavo // Stem Cell Research & Therapy;2010, Vol. 1 Issue 3, p1 

    Derivation of autologous induced pluripotent stem cells (iPSCs) through direct reprogramming of easily accessible somatic cells holds the potential to transform the field of regenerative medicine. Since Takahashi and Yamanaka's groundbreaking study describing the generation of iPSCs by...

  • The H3K27 demethylase Utx regulates somatic and germ cell epigenetic reprogramming. Mansour, Abed AlFatah; Gafni, Ohad; Weinberger, Leehee; Zviran, Asaf; Ayyash, Muneef; Rais, Yoach; Krupalnik, Vladislav; Zerbib, Mirie; Amann-Zalcenstein, Daniela; Maza, Itay; Geula, Shay; Viukov, Sergey; Holtzman, Liad; Pribluda, Ariel; Canaani, Eli; Horn-Saban, Shirley; Amit, Ido; Novershtern, Noa; Hanna, Jacob H. // Nature;8/16/2012, Vol. 488 Issue 7411, p409 

    Induced pluripotent stem cells (iPSCs) can be derived from somatic cells by ectopic expression of different transcription factors, classically Oct4 (also known as Pou5f1), Sox2, Klf4 and Myc (abbreviated as OSKM). This process is accompanied by genome-wide epigenetic changes, but how these...

  • Advances in Culture and Manipulation of Human Pluripotent Stem Cells. Qian, X.; Villa-Diaz, L.G.; Krebsbach, P.H. // Journal of Dental Research;Nov2013, Vol. 92 Issue 11, p956 

    Recent advances in the understanding of pluripotent stem cell biology and emerging technologies to reprogram somatic cells to a stem cell–like state are helping bring stem cell therapies for a range of human disorders closer to clinical reality. Human pluripotent stem cells (hPSCs) have...

  • FOXO1 is an essential regulator of pluripotency in human embryonic stem cells. Zhang, Xin; Yalcin, Safak; Lee, Dung-Fang; Yeh, Tsung-Yin J.; Lee, Seung-Min; Su, Jie; Mungamuri, Sathish Kumar; Rimmel�, Pauline; Kennedy, Marion; Sellers, Rani; Landthaler, Markus; Tuschl, Thomas; Chi, Nai-Wen; Lemischka, Ihor; Keller, Gordon; Ghaffari, Saghi // Nature Cell Biology;Sep2011, Vol. 13 Issue 9, p1092 

    Pluripotency of embryonic stem cells (ESCs) is defined by their ability to differentiate into three germ layers and derivative cell types and is established by an interactive network of proteins including OCT4 (also known as POU5F1; ref. ), NANOG (refs , ), SOX2 (ref. ) and their binding...

  • Oct4 links multiple epigenetic pathways to the pluripotency network. Ding, Junjun; Xu, Huilei; Faiola, Francesco; Ma'ayan, Avi; Wang, Jianlong // Cell Research;Jan2012, Vol. 22 Issue 1, p155 

    Oct4 is a well-known transcription factor that plays fundamental roles in stem cell self-renewal, pluripotency, and somatic cell reprogramming. However, limited information is available on Oct4-associated protein complexes and their intrinsic protein-protein interactions that dictate Oct4's...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics