Scale-up of Human Mesenchymal Stem Cells on Microcarriers in Suspension in a Single-use Bioreactor

Kehoe, Daniel; Schnitzler, Aletta; Simler, Janice; DiLeo, Anthony; Ball, Andrew
March 2012
BioPharm International;Mar2012, Vol. 25 Issue 3, p28
Trade Publication
The article presents a study which demonstrates the utility of collagen-coated microcarriers and 3-L single-use bioreactor for the human bone marrow expansion that is derived with mesenchymal stem cells (MSCs). The study used NucleoCounter, an adipocyte differentiation kit, and reverse transcriptase-polymerase chain reaction (RT-PCR). Results show that the hypoxic condition of the MSCs is confirmed to be closer to the physiological oxygen levels within the bone marrow.


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