Spectrophotometric determination of methylamine moiety containing pesticides using ninhydrine reagent

Khan, Romana; Shah, Jasmin; Rasul Jan, M.
June 2012
Journal of Analytical Chemistry;Jun2012, Vol. 67 Issue 6, p581
Academic Journal
A spectrophotometric method has been proposed for the determination of pesticides containing methylamine moiety. These pesticides afforded methylamine upon alkaline hydrolysis, which was subsequently reacted with ninhydrin in the presence of pyridine. The absorbance of the resulting blue-purple product was measured spectrophotometrically at 570 nm. The Beer's law showed a linear relationship between absorbance and concentration of the pesticide in the range 0.04-5.0 μg/mL. The limit of detection and limit of quantification were 0.012 and 0.02 μg/mL, respectively. The repeatability was 0.22 expressed as RSD. The performance of this newly developed method was compared with the established spectrophotometric methods for the determination of these pesticides. The method has been successfully applied to the evaluation of these pesticides in commercial pesticide formulation and to the determination of monocrotophos in fruit samples.


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