TITLE

Improved Canine and Human Visceral Leishmaniasis Immunodiagnosis Using Combinations of Synthetic Peptides in Enzyme-Linked Immunosorbent Assay

AUTHOR(S)
Costa, Míriam Maria; Penido, Marcos; Dos Santos, Mariana Silva; Doro, Daniel; de Freitas, Eloísa; Michalick, Marilene Susan Marques; Grimaldi, Gabriel; Gazzinelli, Ricardo Tostes; Fernandes, Ana Paula
PUB. DATE
May 2012
SOURCE
PLoS Neglected Tropical Diseases;May2012, Vol. 6 Issue 5, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Zoonotic visceral leishmaniasis (VL) is a severe infectious disease caused by protozoan parasites of the genus Leishmania and the domestic dogs are the main urban parasite reservoir hosts. In Brazil, indirect fluorescence antibody tests (IFAT) and indirect enzyme linked immunosorbent assay (ELISA) using promastigote extracts are widely used in epidemiological surveys. However, their sensitivity and specificity have often been compromised by the use of complex mixtures of antigens, which reduces their accuracy allowing the maintenance of infected animals that favors transmission to humans. In this context, the use of combinations of defined peptides appears favorable. Therefore, they were tested by combinations of five peptides derived from the previously described Leishmania diagnostic antigens A2, NH, LACK and K39. Methodology/Principal Findings: Combinations of peptides derived A2, NH, LACK and K39 antigens were used in ELISA with sera from 44 human patients and 106 dogs. Improved sensitivities and specificities, close to 100%, were obtained for both sera of patients and dogs. Moreover, high sensitivity and specificity were observed even for canine sera presenting low IFAT anti-Leishmania antibody titers or from asymptomatic animals. Conclusions/Significance: The use of combinations of B cell predicted synthetic peptides derived from antigens A2, NH, LACK and K39 may provide an alternative for improved sensitivities and specificities for immunodiagnostic assays of VL.
ACCESSION #
76479744

 

Related Articles

  • Risk factors associated with asymptomatic infection by Leishmania chagasi in north-east Brazil Caldas, A.J.M.; Costa, J.M.L.; Silva, A.A.M.; Vinhas, V.; Barral, A. // Transactions of the Royal Society of Tropical Medicine & Hygiene;Jan2002, Vol. 96 Issue 1, p21 

    Various factors have been associated with a predisposition to the development of clinical American visceral leishmaniasis (AVL). However, little information is available about the factors that predispose to asymptomatic infection. To identify the risk factors associated with asymptomatic...

  • Prevalence of antibodies against three species of Leishmania (L. mexicana, L. braziliensis, L. infantum) and possible associated factors in dogs from Mérida, Yucatán, Mexico Arjona-Jiménez, Guadalupe; Villegas, Noelia; López-Céspedes, Ángeles; Marín, Clotilde; Longoni, Silvia S.; Bolio-González, Manuel E.; Rodríguez-Vivas, Roger I.; Sauri-Arceo, Carlos H.; Sánchez-Moreno, Manuel // Transactions of the Royal Society of Tropical Medicine & Hygiene;Apr2012, Vol. 106 Issue 4, p252 

    Summary: Leishmania spp. has been recorded in humans and in dogs, and numerous studies have demonstrated that dogs act as reservoirs for visceral leishmaniasis. The objective of this study was to determine the prevalence of three species of the Leishmania genus and possible associated factors in...

  • Inaccuracy of Enzyme-Linked Immunosorbent Assay Using Soluble and Recombinant Antigens to Detect Asymptomatic Infection by Leishmania infantum. Moreno, Elizabeth Castro; Gonçalves, Andréa Vieira; Chaves, Anderson Vieira; Melo, Maria Norma; Lambertucci, José Roberto; Andrade, Antero Silva Ribeiro; Deborah Negrão-Corrêa; Antunes, Carlos Mauricio de Figueiredo; Carneiro, Mariângela // PLoS Neglected Tropical Diseases;Oct2009, Vol. 3 Issue 10, p1 

    Background: One of the most important drawbacks in visceral leishmaniasis (VL) population studies is the difficulty of diagnosing asymptomatic carriers. The aim of this study, conducted in an urban area in the Southeast of Brazil, was to evaluate the performance of serology to identify...

  • The Investigation of Antibody Existence Against Leishmania Parasites in Patient Sera Infected by Different Antibodies. NOVRUZOVA, M. S.; BAĞIROVA, M. İ.; CANIM, S.; QURBANOV, A. Е.; ALLAHVERDIYEV, A. M. // International Journal of Natural & Engineering Sciences;May2009, Vol. 3 Issue 2, p45 

    Leishmania parasites are the causal agents of leishmaniasis; a group of protozoan diseases transmitted to mammals, including human beings, by phlebotomizes sandflies. It is known that 12 million people are infected with Leishmania in the world and approximately 350 million people are at a risk...

  • Validity and Reliability of Enzyme Immunoassays Using Leishmania major or L. infantum Antigens for the Diagnosis of Canine Visceral Leishmaniasis in Brazil. de Arruda, Mauro Maciel; Figueiredo, Fabiano Borges; Cardoso, Fernanda Alvarenga; Hiamamoto, Roberto Mitsuyoshi; Brazuna, Júlia Cristina Macksoud; de Oliveira, Maria Regina Fernandes; Noronha, Elza Ferreira; Romero, Gustavo Adolfo Sierra // PLoS ONE;Jul2013, Vol. 8 Issue 7, p1 

    Background: American visceral leishmaniasis is caused by the protozoan Leishmania infantum. Dogs are the main reservoirs in the domestic transmission cycle. The limited accuracy of diagnostic tests for canine leishmaniasis may contribute to the lack of impact of control measures recommended by...

  • Serological and molecular survey of Leishmania parasites in apparently healthy dogs in the West Bank, Palestine. Hamarsheh, Omar; Nasereddi, Abedalmajeed; Damaj, Safa; Sawalha, Samir; Al-Jawabreh, Hanan; Azmi, Kifaya; Amro, Ahmad; Ereqat, Suheir; Abdeen, Ziad; Al-Jawabreh, Amer // Parasites & Vectors;2012, Vol. 5 Issue 1, p183 

    Background: Canine visceral leishmaniasis (CVL) is caused by Leishmania infantum in all Mediterranean countries. The Leishmania parasite is transmitted by the bite of a corresponding sand fly vector and primarily maintained in nature by wild and domestic reservoirs, including dogs, foxes and...

  • Public Knowledge about and Detection of Canine Visceral Leishmaniasis in Urban Divinópolis, Brazil. Margonari, Carina; Menezes, Júlia Alves; Rocha, Marcele Neves; Maia, Kamila Nunes; Oliveira, Michael Éder de; Fonseca, Amanda Luisa; de Sousa, Fabrizio Furtado; Castro Ferreira, Eduardo de; Madureira, Ana Paula; Melo, Maria Norma; Soares, Rodrigo Pedro // Journal of Tropical Medicine (16879686);2012, p1 

    Background. Leishmaniases are diseases with a wide spectrumof clinical manifestations including cutaneous (CL) and visceral (VL) forms.Many factors may affect their occurrence and expansion including environmental, geographic, and social conditions. In the past two decades, Divinópolis,Minas...

  • Dengue Infection and Miscarriage: A Prospective Case Control Study. Peng Chiong Tan; May Zaw Soe; Khaing Si Lay; Seok Mui Wang; Sekaran, Shamala Devi; Omar, Siti Zawiah // PLoS Neglected Tropical Diseases;May2012, Vol. 6 Issue 5, p1 

    Background: Dengue is the most prevalent mosquito borne infection worldwide. Vertical transmissions after maternal dengue infection to the fetus and pregnancy losses in relation to dengue illness have been reported. The relationship of dengue to miscarriage is not known. Method: We aimed to...

  • The binding of proteinase 3 antineutrophil cytoplasmic antibodies (PR3-ANCA) varies in different ELISAs. Trevisin M; Neeson P; Savige J // Journal of Clinical Pathology;Mar2004, Vol. 57 Issue 3, p303 

    BACKGROUND: The demonstration of proteinase 3 specific antineutrophil cytoplasmic antibodies (PR3-ANCA), and the estimation of antibody values are useful in the diagnosis and management of patients with Wegener's granulomatosis (WG). However, external quality assessment programmes suggest that...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics