TITLE

Proteomic analysis of the impact of static culturing on the expansion of rat bone marrow mesenchymal stem cells

AUTHOR(S)
Peter, Stefan; Evans, Caroline; Ow, Saw; Scutt, Andy; Wright, Phillip; Biggs, Catherine
PUB. DATE
August 2012
SOURCE
Biotechnology Letters;Aug2012, Vol. 34 Issue 8, p1589
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The clinical potential of mesenchymal stem cells (MSC) in tissue engineering and regenerative medicine is due to their self-renewal, proliferation and multi-lineage differentiation potential. Clinical use requires large cell numbers; which can, theoretically, be generated by ex vivo expansion of plastic adherent, MSC subpopulation, of bone marrow cells (BMC). Effects of serial culture on MSC phenotype were investigated using non-gel based quantitative proteomic methodology for static monolayer cultures of rat BMC. In total, 382 proteins were relatively quantified (≥2 peptides). Nine proteins were up-regulated and seven down-regulated at passage 4 relative to passage 2 ( p ≤ 0.05). We propose that serial culture impacts on MSC expansion (observed decline in colony forming potential and colony size) is through a combination of osteogenic differentiation and ageing/senescence and propose six novel protein biomarkers as candidates for quality control purposes in bioprocessing.
ACCESSION #
78065537

 

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