Replication of Very Virulent Infectious Bursal Disease Virus in the Chicken Mesenchymal Stem Cells

Mohammed, M. H.; Hair-Bejo, M.; Omar, A. R.; Aini, I.
March 2012
Journal of Advanced Medical Research;2012, Vol. 2 Issue 1, p1
Academic Journal
The susceptibility of the chicken mesenchymal stem cells to very virulent infectious bursal disease (vvIIBDV) was characterized after five consecutive passages in chicken mesenchymal stem cells. Virus replication was monitored by cytopathic effect observation, indirect immunoperoxidase, and reverse transcription polymerase chain reaction (RT-PCR). At 48 h post-infection (p.i.) in first passage, the cytopathic effect was characterized by rounding up of cells and monolayer detachment, intracytoplasmic brownish colouration was readily observed by from 24 h p.i onwards, viral RNA from vvIBDV-infected monolayers was demonstrated by RT-PCR. Tissue culture effective dose50 (EID50) was used to measure virus titration performed on chicken mesenchymal stem cells, and the titres in second passage was 106.6 EID50/ml. The results obtained in this study suggested that the chicken mesenchymal stem cells can be used for vvIBDV propagation, and this may trigger new aspects for tissue culture adaptation of such fastidious virus in the future.


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