Pathological Changes in the White Matter after Spinal Contusion Injury in the Rat

Ek, C. Joakim; Habgood, Mark D.; Dennis, Ross; Dziegielewska, Katarzyna M.; Mallard, Carina; Wheaton, Benjamin; Saunders, Norman R.; Combs, Colin
August 2012
PLoS ONE;Aug2012, Vol. 7 Issue 8, Special section p1
Academic Journal
It has been shown previously that after spinal cord injury, the loss of grey matter is relatively faster than loss of white matter suggesting interventions to save white matter tracts offer better therapeutic possibilities. Loss of white matter in and around the injury site is believed to be the main underlying cause for the subsequent loss of neurological functions. In this study we used a series of techniques, including estimations of the number of axons with pathology, immunohistochemistry and mapping of distribution of pathological axons, to better understand the temporal and spatial pathological events in white matter following contusion injury to the rat spinal cord. There was an initial rapid loss of axons with no detectable further loss beyond 1 week after injury. Immunoreactivity for CNPase indicated that changes to oligodendrocytes are rapid, extending to several millimetres away from injury site and preceding much of the axonal loss, giving early prediction of the final volume of white matter that survived. It seems that in juvenile rats the myelination of axons in white matter tracts continues for some time, which has an important bearing on interpretation of our, and previous, studies. The amount of myelin debris and axon pathology progressively decreased with time but could still be observed at 10 weeks after injury, especially at more distant rostral and caudal levels from the injury site. This study provides new methods to assess injuries to spinal cord and indicates that early interventions are needed for the successful sparing of white matter tracts following injury.


Related Articles

  • Effect of Shikonin on Spinal Cord Injury in Rats Via Regulation of HMGB1/TLR4/NF-κB Signaling Pathway. Bi, Yihui; Zhu, Yapeng; Zhang, Mingkai; Zhang, Keke; Hua, Xingyi; Fang, Zheng; Zhou, Jian; Dai, Wenjie; Cui, Yixing; Li, Jun; You, Tao // Cellular Physiology & Biochemistry (Karger AG);Oct2017, Vol. 43 Issue 2, p481 

    Background/Aims: Shikonin, a compound extracted from Zicao, has been demonstrated to hold anti-bacterial, anti-inflammatory, and anti-tumor activities in various diseases and it has been shown to protect human organs from injuries. However, the effect of shikonin on the recovery of spinal cord...

  • Cell Transplantation: Neural stem cell transplants for spinal cord injury maximized by combined, complimentary therapies.  // Biomedical Market Newsletter;4/21/2012, Vol. 21, p1 

    The article focuses on a research, in which researchers have demonstrated the therapeutic potential of transplanted neural stem cells to reverse complex pathological processes following spinal cord injury andmaximized the benefits of neural stem cell transplantation for spinal cord repair in rats.

  • Spatio-Temporal Progression of Grey and White Matter Damage Following Contusion Injury in Rat Spinal Cord. Ek, C. Joakim; Habgood, Mark D.; Callaway, Jennifer K.; Dennis, Ross; Dziegielewska, Katarzyna M.; Johansson, Pia A.; Potter, Ann; Wheaton, Benjamin; Saunders, Norman R. // PLoS ONE;2010, Vol. 5 Issue 8, p1 

    Cellular mechanisms of secondary damage progression following spinal cord injury remain unclear. We have studied the extent of tissue damage from 15 min to 10 weeks after injury using morphological and biochemical estimates of lesion volume and surviving grey and white matter. This has been...

  • Evaluation of Injured Axons Using Two-Photon Excited Fluorescence Microscopy after Spinal Cord Contusion Injury in YFP-H Line Mice. Hideki Horiuchi; Yusuke Oshima; Tadanori Ogata; Tadao Morino; Seiji Matsuda; Hiromasa Miura; Takeshi Imamura // International Journal of Molecular Sciences;Jul2015, Vol. 16 Issue 7, p15785 

    Elucidation of the process of degeneration of injured axons is important for the development of therapeutic modules for the treatment of spinal cord injuries. The aim of this study was to establish a method for time-lapse observation of injured axons in living animals after spinal cord contusion...

  • Isolation and Characterization of Rat Mesenchymal Stem Cells Derived from Granulocyte Colony-Stimulating Factor-Mobilized Peripheral Blood. Fu, Qiang; Zhang, Qian; Jia, Lin Ying; Fang, Ning; Chen, Long; Yu, Li Mei; Liu, Jin Wei; Zhang, Tao // Cells Tissues Organs;Aug2016, Vol. 201 Issue 6, p412 

    Mesenchymal stem cells (MSCs) have been isolated from many tissues and organs. However, there is much dispute as to whether MSCs exist in peripheral blood. This may be due to the limited identification methods of MSCs, especially the lack of detection markers for phenotypic characteristics. In...

  • Label-Free Spectral Imaging Unveils Biochemical Mechanisms of Low-Level Laser Therapy on Spinal Cord Injury. Yuze Gong; Shuang Wang; Zhuowen Liang; Zhe Wang; Xu Zhang; Jie Li; Jiwei Song; Xueyu Hu; Kaige Wang; Qingli He; Jintao Bai // Cellular Physiology & Biochemistry (Karger AG);Oct2018, Vol. 49 Issue 3, p1168 

    Background/Aims: Low-level laser therapy (LLLT) leads to complex photochemical responses during the healing process of spinal cord injury (SCI). Confocal Raman Microspectral Imaging (in combination with multivariate analysis) was adopted to illustrate the underlying biochemical mechanisms of...

  • Fibrin Scaffolds Containing Ectomesenchymal Stem Cells Enhance Behavioral and Histological Improvement in a Rat Model of Spinal Cord Injury. Liu, Jinbo; Chen, Qian; Zhang, Zhijian; Zheng, Yuhua; Sun, Xianglan; Cao, Xudong; Gong, aihua; Cui, Yanhong; He, Qinghua; Jiang, Ping // Cells Tissues Organs;2013, Vol. 198 Issue 1, p35 

    Fibrin has been widely used in wound healing. However, its benefit for spinal cord injury (SCI) is limited. In this study, we investigated the impact of fibrin scaffolds containing ectomesenchymal stem cells (EMSCs) on histological and behavioral recovery after SCI and compared it with fibrin...

  • Ryanodine Receptor 2 Plays a Critical Role in Spinal Cord Injury via Induction of Oxidative Stress. Bo Liao; Yong Zhang; Honghui Sun; Baoan Ma; Jixian Qian // Cellular Physiology & Biochemistry (Karger AG);May2016, Vol. 38 Issue 5, p1129 

    Background/Aims: Spinal cord injury (SCI) is a severe health problem worldwide. Ryanodine receptors (RyRs) are a class of intracellular calcium channels in various excitable tissues such as muscles and nervous tissues. The current study was designed to investigate the possible role of RyR2...

  • The Therapeutic Profile of Rolipram, PDE Target and Mechanism of Action as a Neuroprotectant following Spinal Cord Injury. Schaal, Sandra Marie; Garg, Maneesh Sen; Ghosh, Mousumi; Lovera, Lilie; Lopez, Michael; Patel, Monal; Louro, Jack; Patel, Samik; Tuesta, Luis; Wai-Man Chan; Pearse, Damien Daniel; Ceña, Valentin // PLoS ONE;Sep2012, Vol. 7 Issue 9, Special section p1 

    The extent of damage following spinal cord injury (SCI) can be reduced by various neuroprotective regimens that include maintaining levels of cyclic adenosine monophosphate (cyclic AMP), via administration of the phosphodiesterase 4 (PDE4) inhibitor Rolipram. The current study sought to...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics