TITLE

Co-cultivated mesenchymal stem cells support chondrocytic differentiation of articular chondrocytes

AUTHOR(S)
Zuo, Qiang; Cui, Weiding; Liu, Feng; Wang, Qing; Chen, Zhefeng; Fan, Weimin
PUB. DATE
April 2013
SOURCE
International Orthopaedics;Apr2013, Vol. 37 Issue 4, p747
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: This study investigated which of the reciprocal stimuli between articular chondrocytes (ACs) and mesenchymal stem cells (MSCs) played the more important role in enhancing cartilage matrix formation, and examined the relative importance of physical contact and soluble factors in the co-culture system. Methods: Rat ACs and bone marrow MSCs with green fluorescent protein (GFP-BMSCs) were co-cultured in vitro with or without direct cell-cell contact at the ratio of 2:1. After co-culturing in direct cell-cell contact, ACs and GFP-BMSCs were separated by flow cytometry. The effects of different co-culture methods were analysed by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. Results: SOX-9, COL2 and aggrecan mRNA levels and protein expression in ACs co-cultured with direct cell-cell contact were significantly higher than in ACs co-cultured without direct cell-cell contact; and similar results were found in GFP-BMSCs. After co-culture either with or without direct cell-cell contact, mRNA levels and protein expression of SOX-9, COL2 and aggrecan in GFP-BMSCs were significantly lower than in ACs in the equivalent co-culture systems. Though the expression of chondrocyte-specific proteins in GFP-BMSCs was enhanced, the protein expression was still much lower than in ACs cultured alone. Conclusions: Reciprocal interactions exist between ACs and BMSCs in co-culture. The stimulating and supporting effects of BMSCs on ACs were more important in enhancing cartilage-matrix formation than the reciprocal effect of ACs on BMSCs. Both soluble factors and direct physical contact occur in AC/BMSC co-cultures, with physical contact playing a predominant, or at least very important role.
ACCESSION #
86406690

 

Related Articles

  • Effects of lentiviral infection of mesenchymal stem cells on the expression of octamer transcription factor 4. JING CHANG; LI TANG; HAN LEI; XIAO-GANG ZHANG; ZHONG ZUO; WEI HUANG; HANG FU // Molecular Medicine Reports;2014, Vol. 10 Issue 5, p2249 

    The present study aimed to investigate the effects of lentiviral infection of human umbilical cord mesenchymal stem cells (hUCMSCs) on the expression of octamer transcription factor 4 (Oct4). hUCMSCs were infected with lentivirus carrying the green fluorescent protein gene (GFP) at different...

  • Hypertonic conditions enhance cartilage formation in scaffold-free primary chondrocyte cultures. Ylärinne, Janne; Qu, Chengjuan; Lammi, Mikko // Cell & Tissue Research;Nov2014, Vol. 358 Issue 2, p541 

    The potential of hypertonic conditions at in vivo levels to promote cartilage extracellular matrix accumulation in scaffold-free primary chondrocyte cultures was investigated. Six million bovine primary chondrocytes were cultured in transwell inserts in low glucose (LG), high glucose (HG), or...

  • Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential. Yuichiro Yamaguchi; Jun Ohno; Ayako Sato; Hirofumi Kido; Tadao Fukushima // BMC Biotechnology;2014, Vol. 14 Issue 1, p127 

    Background Mesenchymal stem cells (MSCs) are a favored cell source for regenerative medicine because of their multilinage potential. However, the conventional monolayer technique used to culture MSCs, inadequately overcomes their low differentiation capacity. Culture of MSCs in multicellular...

  • Gene Expression Profiles of Human Adipose Tissue-Derived Mesenchymal Stem Cells Are Modified by Cell Culture Density. Kim, Dae Seong; Lee, Myoung Woo; Yoo, Keon Hee; Lee, Tae-Hee; Kim, Hye Jin; Jang, In Keun; Chun, Yong Hoon; Kim, Hyung Joon; Park, Seung Jo; Lee, Soo Hyun; Son, Meong Hi; Jung, Hye Lim; Sung, Ki Woong; Koo, Hong Hoe // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    Previous studies conducted cell expansion ex vivo using low initial plating densities for optimal expansion and subsequent differentiation of mesenchymal stem cells (MSCs). However, MSC populations are heterogeneous and culture conditions can affect the characteristics of MSCs. In this study,...

  • Isolation and in vitro expansion of Lgr6-positive multipotent hair follicle stem cells. Nath, Miriam; Offers, Monika; Hummel, Michaela; Seissler, Jochen // Cell & Tissue Research;Jun2011, Vol. 344 Issue 3, p435 

    Hair follicles (HFs) are a well-known niche of multipotent stem cells. Recently, HF cells expressing leucine-rich orphan G protein-coupled receptors (Lgr) have been described as multipotent adult stem cells with a high potential for regenerative therapies. We have now established the conditions...

  • Hypoxic adipose mesenchymal stem cells derived conditioned medium protects myocardial infarct in rat. HE, J.; CAI, Y.; LUO, L.-M.; LIU, H.-B. // European Review for Medical & Pharmacological Sciences;Nov2015, Vol. 19 Issue 22, p4397 

    OBJECTIVE: The aim of this study was to explore the impact of normoxic and hypoxic cell-culture conditions on the expression and secretion of adipose mesenchymal stem cells (ADMSCs)-derived paracrine molecules, and to evaluate the cardioprotective role of hypoxic condition medium (hypoCM) in...

  • Five percent oxygen tension is not beneficial for neocartilage formation in scaffold-free cell cultures. Qu, Chengjuan; Lindeberg, Heli; Ylärinne, Janne; Lammi, Mikko // Cell & Tissue Research;Apr2012, Vol. 348 Issue 1, p109 

    We have investigated whether 5% oxygen tension (O) is beneficial for neocartilage formation when chondrocytes are cultured in transwell-COL inserts. Six million bovine primary chondrocytes were cultured in an insert with DMEM supplemented with 10% fetal bovine serum and antibiotics, with or...

  • Influence of extracellular matrix on the expression of inflammatory cytokines, proteases, and apoptosis-related genes induced by hydrostatic pressure in three-dimensionally cultured chondrocytes. Taisuke Kunitomo; Kenji Takahashi; Yuji Arai; Kei Sakao; Kuniaki Honjo; Masazumi Saito; Atsuo Inoue; Hitoshi Tonomura; Toru Morihara; Osam Mazda; Jiro Imanishi; Toshikazu Kubo // Journal of Orthopaedic Science;Nov2009, Vol. 14 Issue 6, p776 

    Abstract Background  The purpose of this study was to investigate the influence of hydrostatic pressure (HP) on the gene expression of cartilage matrix, cytokines, and apoptosis-associated factors in chondrocytes in which the cartilage was in extracellular matrix (ECM)-rich or ECM-poor...

  • Replication of Very Virulent Infectious Bursal Disease Virus in the Chicken Mesenchymal Stem Cells. Mohammed, M. H.; Hair-Bejo, M.; Omar, A. R.; Aini, I. // Journal of Advanced Medical Research;2012, Vol. 2 Issue 1, p1 

    The susceptibility of the chicken mesenchymal stem cells to very virulent infectious bursal disease (vvIIBDV) was characterized after five consecutive passages in chicken mesenchymal stem cells. Virus replication was monitored by cytopathic effect observation, indirect immunoperoxidase, and...

Share

Other Topics