TITLE

Preparation of progenin III from total steroidal saponins of Dioscorea nipponica Makino using a crude enzyme from Aspergillus oryzae strain

AUTHOR(S)
Liu, Tingqiang; Yu, Hongshan; Liu, Chunying; Bao, Yongming; Hu, Xiangchun; Wang, Yuanhao; Liu, Bing; Fu, Yaoyao; Tang, Sihui; Jin, Fengxie
PUB. DATE
May 2013
SOURCE
Journal of Industrial Microbiology & Biotechnology;May2013, Vol. 40 Issue 5, p427
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Progenin III, one of the most active spirostanol saponins, is a potential candidate for anti-cancer therapy due to its strong antitumor activity and low hemolytic activity. However, the concentration of progenin III is extremely low in natural Dioscorea plants. In this paper, the progenin III production from total steroidal saponins of Dioscorea nipponica Makino was studied using the crude enzyme from Aspergillus oryzae DLFCC-38. The crude enzyme converting total steroidal saponins into progenin III was obtained from the A. oryzae DLFCC-38 culture. For enzyme production, the strain was cultured for 72 h at 30 °C with shaking at 150 rpm in 5 % (w/v) malt extract medium containing 2 % (v/v) extract of D. nipponica as the enzyme inducer. The crude enzyme converted total steroidal saponins into major progenin III with a high yield when the reaction was carried out for 9 h at 50 °C and pH 5.0 with the 20 mg/ml of substrate. In the preparation of progenin III, 117 g of crude progenin III was obtained from 160 g of substrate, and the crude product was purified with silica gel column to obtain 60.3 g progenin III of 93.4 % purity.
ACCESSION #
86976965

 

Related Articles

  • Membrane Disintegration Caused by the Steroid Saponin Digitonin Is Related to the Presence of Cholesterol. Sudji, Ikhwan Resmala; Subburaj, Yamunadevi; Frenkel, Nataliya; García-Sáez, Ana J.; Wink, Michael // Molecules;2015, Vol. 20 Issue 11, p20146 

    In the present investigation we studied the molecular mechanisms of the monodesmosidic saponin digitonin on natural and artificial membranes. We measured the hemolytic activity of digitonin on red blood cells (RBCs). Also different lipid membrane models (large unilamellar vesicles, LUVs, and...

  • Microvascular mechanisms by which the combretastatin A-4 derivative AC7700 (AVE8062) induces tumour blood flow stasis. Hori, K.; Saito, S. // British Journal of Cancer;10/6/2003, Vol. 89 Issue 7, p1334 

    We previously reported that a novel combretastatin A-4 derivative, AC7700, has remarkable antitumour effects because of an irreversible stasis of tumour blood flow (TBF) and subsequent loss of nutrient supply to tumour tissue. Since early 2002, under the new designation AVE8062, AC7700 has...

  • Steroidal Saponins and Flavonoids from Ornithogalum thyrsoides. Cao Xiu-Ping; Zou Jian-Hua; Qiu De-You; Dai Jun-Gui // Natural Product Research & Development;Jun2012, Vol. 24 Issue 6, p768 

    Seven compounds were isolated and purified by sihca gel, Sephadex LH-20, ODS and semi-preparative HPLC from ethyl acetate extraction of Ornithogalum thyrsoides. By means of extensive spectroscopic data, their structures were identified as (23S, 24S, 25S) -23,24-dihydroxyruscogenin...

  • Synthesis and Antitumor Evaluation of (E)-2-Benzothiazole Hydrazones. Nogueira, Ant�nio Francisco; Azevedo, Elisa Carvalho; Ferreira, Vitor Francisco; Ara�jo, Ana J�rsia; Dos Santos, Evelyne Alves; Pessoa, Claudia; Costa-Lotufo, Let�cia Veras; Montenegro, Raquel Carvalho; de Moraes, Manoel Odorico; Vasconcelos, Thatyana Rocha Alves // Letters in Drug Design & Discovery;Oct2010, Vol. 7 Issue 8, p551 

    No abstract available.

  • Late spring characterization of different coastal areas of the Adriatic Sea. MARINI, Mauro; CAMPANELLI, Alessandra; SANXHAKU, Mitat; KLJAJIĆ, Zoran; BETTI, Mattia; GRILLE, Federica // Acta Adriatica;2015, Vol. 56 Issue 1, p27 

    The objective of this study is to analyze the physical and chemical characteristics of three coastal zones of the Adriatic Sea during late spring, and to identify similarities and differences among the zones. The trophic status of the Southeastern Adriatic-Sea, dominated by the discharge from...

  • Glycyrrhiza glabra and Glycyrrhiza echinata - Sources of Low Hemotoxic Saponins. Carmen, Canciu-Dobrea; Adriana, Kaycsa; Cristina, Dehelean; Codruţa, Şoica; Diana, Antal // Acta Medica Marisiensis;2012, Vol. 58 Issue 3, p150 

    Introduction: Glycyrrhiza glabra L. (licorice) is one of the most used medicinal plats as an expectorant, anti-inflammatory, anti-ulcer, sweetener, antioxidant, antibacterial, antiviral, antimycotic or anti-tumor agent. In our country G. echinata (Russian licorice) is the dominant species of the...

  • Ulososides and Urabosides - Triterpenoid Saponins from the Caribbean Marine Sponge Ectyoplasia ferox. Colorado, Jhonny; Muñoz, Diana; Marquez, Diana; Marquez, Maria Elena; Lopez, Juan; Thomas, Olivier P.; Martinez, Alejandro // Molecules;Mar2013, Vol. 18 Issue 3, p2598 

    Three new triterpene glycosides, named ulososide F (1), urabosides A (2) and B (3), together with the previously reported ulososide A (4), were isolated from the Caribbean marine sponge Ectyoplasia ferox. Their structures were elucidated using extensive interpretation of 1D and 2D-NMR data, as...

  • The importance of hemolysis in the lethal effects of equnatoxin II, a protein from the sea anemone Actinia equina (L.). Bunc, Matjaž; Bregar, Renata; Šuput, Dušan // Pflugers Archiv European Journal of Physiology;Oct2000, Vol. 440 Issue 7, pr151 

    Equinatoxin II (EqT II) is one of the three isotoxins isolated from the sea anemone Actinia equina (L.). The cause of death due to i.v. application of Eq T II was attributed to its hemolytic activity and hiperkaliemia, and the direct cardio-respiratory effects of the toxin. The toxin also binds...

  • Long lasting remission by rituximab in a patient with primary cold agglutinin autoimmune haemolytic anaemia. Glaser, Marjana; Glaser, Andrej; Skalicky, Marjan // Wiener Klinische Wochenschrift;Nov2011, Vol. 123 Issue 21/22, p680 

    A 76-year-old female patient with cold agglutinin autoimmune haemolytic anaemia (CAHA) is presented. The haemolysis worsened during infection. Earlier, the patient underwent an unsuccessful treatment with steroids, chlorambucil , folic acid and heated erythrocyte transfusion. We treated her with...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics