April 2013
International Journal of Pharmacy & Pharmaceutical Sciences;2013 Supplement 2, Vol. 5, p172
Academic Journal
Objective: Nowadays there is an increasing interest to extract and isolate naturally antioxidant compounds especially phenolic compounds that are pharmacologically potent and play an important role as a health-protecting factor. They neutralize the free radicals, which are unstable molecules that are linked with the development of a number of degenerative diseases. This study aims to investigate the antioxidant properties and phenolic compounds from the leaves of Gleditsia triacanthos L. (Family Leguminosae). Methods: The 85% methanol extract of the leaves of Gleditsia triacanthos L. and the chloroform and ethyl acetate fractions were estimated for total antioxidant capacity, 1,1-diphenyl picrylhydrazyl (DPPH) free radical scavenging, total phenolic and flavonoid contents using spectrophotometric methods. The ethyl acetate and chloroform fractions were subjected to chromatographic and spectroscopic analysis. Results: The results showed that the highest content of phenols and flavonoids were found in methanol extract and ethyl acetate fraction whereas the lowest content was recorded in the chloroform fraction and there is correlation between the total phenolic content and the antioxidant activity of these extracts. Also, five compounds have been isolated from the chloroform and the ethyl acetate fractions and their structures have been elucidated as caffeic acid, luteolin, isovitexin, apigenin-7-O-β-D-glucopyranoside and Luteolin-7-O-β-D-glucopyranoside. Evaluation of the five isolated compounds as antioxidant agent against DPPH-radical revealed that the five compounds have antioxidant activity (SC50 = 9.53, 11.67, 22.45, 98.44 and 58.91 μg/ml respectively). Recommendation: This plant extract could be used as a natural antioxidant after comprehensive in vitro and in vivo biological studies.


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