TITLE

EVALUATION OF THE SENSITIVITY AND OVER-EXPRESSION OF THE GENES CDR1 AND MDR1 TO FLUCONAZOLE IN CLINICAL CANDIDA ALBICANS ISOLATES FROM IRANIAN ORAL CANDIDIASIS AIDS PATIENTS

AUTHOR(S)
Armaki, Mojtaba Taghizadeh; Yadegari, Mohammad Hossein; Bazl, Masoumeh Rajabi; Farahbakhsh, Ehsan
PUB. DATE
July 2013
SOURCE
Jundishapur Journal of Microbiology;2013 Special Edition, p3
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Introduction and Objectives: Fluconazole-resistant Candida albicans is the cause of oral candidiasis in patients with human immunodeficiency virus infection (HIV). The molecular mechanisms of azole resistance in C. albicans include increased levels of expression efflux transporters MDR1 and CDR1 and alterations in the target enzyme. Materials and Methods: The in vitro activities of fluconazole against 66 isolates of C. albicans were examined. The susceptibility of the antifungal drugs was determined by broth macrodilution and disk diffusion methods according to the Clinical and Laboratory Standards Institute (CLSI). Total RNA was extracted from, drug resistant and sensitive C. albicans. cDNA was synthesized by using the MULV Reverse Transcirptase. Semi quantitative RT-PCR was used to measure expression levels of the genes encoding efflux pumps MDR1 and CDR1 and control gene (ACT1) in 66 isolates of C. albicans. Results: Of the 66 isolates tested with disk diffusion, 45 (68.18%) isolates were susceptible to fluconazole, 5 (7.58%) were susceptible dose-dependent and 16 (24.24%) were resistant to fluconazole. However, in CLSI broth macrodilution test, of the 66 isolates tested, 43 (65.15%) isolates were susceptible, 8 (12.12%) were susceptible dose-dependent and 15 (22.73%) were resistant to fluconazole. Finally, MDR1 and CDR1 genes were overexpressed in 2 (3.03%) and 8 (12.12%) clinical isolates of C. albicans, respectively. Discussion: Nowadays, many advanced and extensive methods are used in reference laboratories to reveal molecular mechanisms beyond drug resistances but Sq-RT-PCR has higher sensitivity relative to Southern blot, Northern blot, general PCR and SDS-PAGE method.
ACCESSION #
89588530

 

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